Humans Surviving Cholera Develop Antibodies against Vibrio cholerae O-Specific Polysaccharide That Inhibit Pathogen Motility.

MBio
Richelle C CharlesEdward T Ryan

Abstract

The mechanism of protection against cholera afforded by previous illness or vaccination is currently unknown. We have recently shown that antibodies targeting O-specific polysaccharide (OSP) of Vibrio cholerae correlate highly with protection against cholera. V. cholerae is highly motile and possesses a flagellum sheathed in OSP, and motility of V. cholerae correlates with virulence. Using high-speed video microscopy and building upon previous animal-related work, we demonstrate that sera, polyclonal antibody fractions, and OSP-specific monoclonal antibodies recovered from humans surviving cholera block V. cholerae motility at both subagglutinating and agglutinating concentrations. This antimotility effect is reversed by preadsorbing sera and polyclonal antibody fractions with purified OSP and is associated with OSP-specific but not flagellin-specific monoclonal antibodies. Fab fragments of OSP-specific polyclonal antibodies do not inhibit motility, suggesting a requirement for antibody-mediated cross-linking in motility inhibition. We show that OSP-specific antibodies do not directly affect V. cholerae viability, but that OSP-specific monoclonal antibody highly protects against death in the murine cholera model. We used in viv...Continue Reading

References

Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·C C Häse, J J Mekalanos
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jun ZhuJohn J Mekalanos
Jun 7, 2002·Nature·D Scott MerrellAndrew Camilli
Aug 5, 2005·Nature·Katia KoelleGolam Mostafa
Oct 24, 2006·PLoS Pathogens·Alex Toftgaard NielsenGary K Schoolnik
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Eric J NelsonStephen B Calderwood
Jun 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·D Ewen CameronJohn J Mekalanos
Jul 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Zhi LiuJun Zhu
Sep 1, 2009·Journal of Bacteriology·Khalid Ali SyedKarl E Klose
Jul 30, 2011·Current Opinion in Infectious Diseases·Richelle C Charles, Edward T Ryan
May 3, 2014·PLoS Neglected Tropical Diseases·Richelle C CharlesJason B Harris
Jun 5, 2015·PLoS Neglected Tropical Diseases·Mohammad AliDavid A Sack
May 10, 2017·Journal of Bacteriology·Alyssa S BallJulia C van Kessel
Jul 22, 2017·The Lancet Infectious Diseases·Qifang BiUNKNOWN Oral Cholera Vaccine Working Group of The Global Task Force on Cholera Control
Mar 20, 2019·Applied Microbiology and Biotechnology·Manyu LuoHuili Lu
Nov 30, 2019·MSphere·Ana A WeilJason B Harris
Aug 21, 2020·Applied Microbiology and Biotechnology·Fazlurrahman KhanYoung-Mog Kim

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
Scanning electron microscopy
ELISA

Software Mentioned

MetaMorph

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.