Humic Substances Alter Ammonia Production and the Microbial Populations Within a RUSITEC Fed a Mixed Hay - Concentrate Diet

Frontiers in Microbiology
S A TerryA V Chaves

Abstract

Humic substances are a novel feed additive which may have the potential to mitigate enteric methane (CH4) production from ruminants as well as enhance microbial activity in the rumen. The aim of this study was to examine the effects of humic substances on fermentation characteristics and microbial communities using the rumen stimulation technique (RUSITEC). The experiment was conducted as a completely randomized design with 3 treatments duplicated in 2 runs (a 15-day period each run) with 2 replicates per run. Treatments consisted of a control diet (forage:concentrate; 60:40) without humic substances or humic substances added at either 1.5 g/d or 3.0 g/d. Dry matter disappearance, pH, fermentation parameters and gas production were measured from day 8 to 15. Samples for microbial profiling were taken on day 5, 10, and 15 using the digested feed bags for solid- associated microbes (SAM) and fermenter fluid for liquid- associated microbes (LAM). The inclusion of humic substances had no effect (P ≥ 0.19) on DM disappearance, pH or the concentrations of VFA. The production of NH3 was linearly decreased (P = 0.04) with increasing levels of humic substances in the diet. There was no effect (P ≥ 0.43) of humic substances on total gas,...Continue Reading

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Citations

Dec 6, 2019·Frontiers in Microbiology·Daniel L ForwoodAlex V Chaves
Aug 1, 2020·Translational Animal Science·Stephanie A TerryTim A McAllister
Nov 24, 2020·Frontiers in Microbiology·Lucy ZhaoAlex V Chaves

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Datasets Mentioned

BETA
PRJNA436853

Methods Mentioned

BETA
PCR

Software Mentioned

R package vegan
RUSITEC
vegan
SAS
QIIME
PERMANOVA

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