Humic substances as electron acceptors for anaerobic oxidation of methane driven by ANME-2d

Water Research
Ya-Nan BaiRaymond J Zeng

Abstract

Humic substances (humics) are ubiquitous in terrestrial and aquatic environments where they can serve as electron acceptors for anaerobic oxidation of organic compounds. Methane is a powerful greenhouse gas, as well as the least reactive organic molecule. Anaerobic oxidation of methane (AOM) coupled to microbial reduction of various electron acceptors plays a crucial role in mitigating methane emissions. Here, we reported that humics could serve as terminal electron acceptors for AOM using enriched nitrate-reducing AOM microorganisms. AOM coupled to the reduction of humics was demonstrated based on the production of 13C-labelled carbon dioxide, and AOM activity was evaluated with different methane partial pressures and electron acceptor concentrations. After three-cycle reduction, both AOM activity and copy numbers of the archaea 16S rRNA and mcrA genes were the highest when anthraquinone-2,6-disulfonic acid and anthraquinone-2-sulfonic acid were electron acceptors. The high-throughput sequencing results suggested that ANME-2d were the dominant methane oxidation archaea after humics reduction, although the partner bacteria NC10 trended downward, other reported humics reduction bacteria (Geobactor and Anammox) appeared. The pote...Continue Reading

Citations

May 1, 2020·Frontiers in Microbiology·Edgardo I ValenzuelaFrancisco J Cervantes
Jun 25, 2020·Environmental Science and Pollution Research International·Fan LuoLinda Sarpong
Nov 4, 2020·Chemosphere·Duyen Minh PhamArata Katayama
Mar 9, 2021·Frontiers in Microbiology·Anna J WalleniusMike S M Jetten
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natalia A Kulikova, Irina V Perminova
Aug 14, 2021·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Mingmei ChiYanling Qiu
Jun 12, 2020·Environmental Science & Technology·Hefei WangLizhong Zhu
Sep 16, 2021·Environmental Microbiology Reports·Xueqin ZhangShihu Hu
Jun 5, 2020·The Science of the Total Environment·Léa CabrolMaialen Barret

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