Humicola insolens cutinase-catalyzed lactone ring-opening polymerizations: kinetic and mechanistic studies

Biomacromolecules
Mo HunsenRichard A Gross

Abstract

This paper explores reaction kinetics and mechanism for immobilized Humicola insolenscutinase (HIC), an important new biocatalyst that efficiently catalyzes non-natural polyester synthetic reactions. HIC, immobilized on Lewatit, was used as catalyst for epsilon-caprolactone (CL) and omega-pentadecalactone (PDL) ring-opening polymerizations (ROPs). Plots of percent CL conversion vs time were obtained in the temperature range from 50 to 90 degrees C. The kinetic plot of ln([M]0/[M]t) vs time (r2 = 0.99) for HIC-catalyzed bulk ROP of CL was linear, indicating that chain termination did not occur and the propagation rate is first order with respect to monomer concentration. Furthermore, linearity to 90% conversion for M(n) vs fractional CL conversion is consistent with a chain-end propagation mechanism. Deviation from linearity above 90% conversion indicates that a competition between ring-opening chain-end propagation and chain growth by steplike polycondensations takes place at high monomer conversion. HIC was inactive for catalysis of L-lactide and (R,S)-beta-butyrolactone ROP. HIC-catalyzed ROP of epsilon-CL and PDL in toluene were successfully performed, giving high molecular weight poly(epsilon-caprolactone) and omega-poly(pe...Continue Reading

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jul 23, 1999·International Journal of Biological Macromolecules·S NamekawaS Kobayashi
Nov 11, 1999·Biotechnology and Bioengineering·C M CarvalhoJ M Cabral
Nov 26, 1999·Biochimica Et Biophysica Acta·S Longhi, C Cambillau
Nov 17, 2001·Chemical Reviews·R A GrossB Kalra
Dec 13, 2001·Chemical Reviews·S KobayashiS Kimura
Feb 9, 2002·Science·Larry L Hench, Julia M Polak
Feb 13, 2007·Small·Prashanth AsuriJonathan S Dordick
Jun 5, 2007·Journal of Biotechnology·Johnathan T GorkeFriedrich Srienc
May 30, 1980·Science·P E Kolattukudy

❮ Previous
Next ❯

Citations

Jan 26, 2016·Trends in Biotechnology·Alessandro PellisLucia Gardossi
Jan 23, 2016·Chemical Reviews·Shin-ichiro ShodaShiro Kobayashi
May 15, 2012·Enzyme and Microbial Technology·Christian KazenwadelKatja Koschorreck
Oct 18, 2008·Macromolecular Bioscience·Marc HansMartin Moeller
Apr 13, 2017·Microbial Biotechnology·Ren Wei, Wolfgang Zimmermann
Jan 1, 2018·Journal of Chemical Technology and Biotechnology·Hua Zhao
Jun 12, 2018·Enzyme and Microbial Technology·An SuRichard Gross
Jan 26, 2010·Biomacromolecules·David Feder, Richard A Gross
Oct 15, 2009·Chemical Reviews·Shiro Kobayashi, Akira Makino

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.