Humoral and bronchial immune responses in cattle experimentally infected with Mycoplasma mycoides subsp. mycoides small colony type

Veterinary Microbiology
Abdo el-MJoachim Frey

Abstract

The course of immune reactions of the manifold antigens of Mycoplasma mycoides subsp. mycoides small colony type (SC) was analysed in serum and bronchial lavage of cattle experimentally infected with the African strain Afadé and the European strain L2 using Western-blots and complement fixation. Western-blot analysis of total antigens of both strains with sera from animals infected with the homologous and heterologous strain revealed the common dominant immunogenic antigens with the molecular masses of 110, 95, 85, 80, 72, 62, 48 and 39 kDa. The sequential sampling of the blood and bronchial lavages before and after contact infections allowed us to identify the antigens of 85, 80, 72, 48 and 39 kDa as particularly early immunogens. The IgA Western blots of the bronchial lavages showed distinct, early and persistent reactions to the 110, 85, 80, 72, 48 and 45 kDa proteins. These proteins were the predominant lipoproteins as determined by [14C]palmitic acid labelling. Only relatively weak reactions of the bronchial lavages were detected with IgG. In general immune responses were significantly stronger in the animals infected with the African strain Afadé, which gave positive results two weeks after contact infection. In contrast,...Continue Reading

References

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Citations

Aug 4, 2007·Antonie van Leeuwenhoek·Laetitia Bonvin-KlotzMichael H Stoffel
Apr 18, 2002·FEMS Microbiology Letters·Joakim WestbergKarl Erik Johansson
Oct 18, 2002·Veterinary Microbiology·José M Martin AlonsoFrancisco Parra
Dec 22, 1999·Trends in Microbiology·I ChambaudA Blanchard
Nov 14, 2007·Infection and Immunity·Daniela F BischofJoachim Frey
Jun 12, 2002·Journal of Bacteriology·Anja PerssonFrançois Poumarat
Jun 8, 2010·BMC Veterinary Research·Myriam ChazelFrançois Poumarat
Jan 26, 2016·Microbial Pathogenesis·Yumei ZhouJiuqing Xin
May 31, 2011·Preventive Veterinary Medicine·Florence TardyFrançois Poumarat
Feb 4, 2009·Comparative Immunology, Microbiology and Infectious Diseases·Laurence DedieuJoachim Frey
Dec 13, 2006·The Veterinary Journal·Paola PiloEdy M Vilei
Jul 7, 2000·Clinical and Diagnostic Laboratory Immunology·E M AbdoJ Frey
May 12, 2020·Veterinary Pathology·Giovanni Di TeodoroMassimo Scacchia

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