Humoral and cellular CMV responses in healthy donors; identification of a frequent population of CMV-specific, CD4+ T cells in seronegative donors.

PloS One
Nina LoethAnette Stryhn

Abstract

CMV status is an important risk factor in immune compromised patients. In hematopoeitic cell transplantations (HCT), both donor and recipient are tested routinely for CMV status by serological assays; however, one might argue that it might also be of relevance to examine CMV status by cellular (i.e., T lymphocyte) assays. Here, we have analyzed the CMV status of 100 healthy blood bank donors using both serology and cellular assays. About half (56%) were found to be CMV seropositive, and they all mounted strong CD8+ and/or moderate CD4+ T cell responses ex vivo against the immunodominant CMV protein, pp65. Of the 44 seronegative donors, only five (11%) mounted ex vivo T cell responses; surprisingly, 33 (75%) mounted strong CD4+ T cell responses after a brief in vitro peptide stimulation culture. This may have significant implications for the analysis and selection of HCT donors.

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Citations

Jul 31, 2013·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Cinja SukdolakBritta Eiz-Vesper
Apr 24, 2015·Human Vaccines & Immunotherapeutics·Benson OgunjimiPhilippe Beutels
Jan 17, 2014·Clinical and Vaccine Immunology : CVI·Benson OgunjimiPierre Van Damme
Oct 26, 2018·Reviews in Medical Virology·David NavarroPilar Pérez-Romero
Jul 5, 2018·Journal of Translational Medicine·Zhenjiang LiuMarkus Maeurer
Jul 23, 2016·Journal of Medical Virology·Edith ReuschelBirgit Seelbach-Goebel

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
density gradient centrifugation
FACS
flow cytometry

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