Humoral and cellular immunities elicited by HIV-1 vaccination

Journal of Pharmaceutical Sciences
J W ShiverM A Liu

Abstract

Recently it has been shown that immunization with plasmid DNA encoding genes for viral or bacterial antigens can elicit both humoral and cellular immune responses in rodents and nonhuman primates. In this study, mice and nonhuman primates were vaccinated by intramuscular injection with plasmids that express either a secreted form of HIV-1 gp120 or rev proteins. Mice receiving the tPA-gp120 DNA developed antigen-specific antibody responses against recombinant gp120 protein and the V2 peptide neutralization epitope as determined by ELISA. Vaccinated mice also exhibited gp120-specific T cell responses, such as in vitro proliferation of splenocytes and MHC Class I-restricted cytotoxic T lymphocyte (CTL) activities, following antigen restimulation. In addition, supernatants from these lymphocyte cultures showed high levels of gamma-interferon production compared with IL-4, suggesting that primarily type 1-like helper T (Th1) lymphocyte responses were induced by both vaccines. Th1-like responses were also obtained for mice vaccinated with rev DNA. Immune responses induced by gp120 or rev vaccines were dose-dependent, boostable, and long-lived (> or = 6 months). Nonhuman primates vaccinated with tPA-gp120 DNA also showed antigen-speci...Continue Reading

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Citations

Jan 1, 1997·Pharmacology & Therapeutics·D L MontgomeryM A Liu
Jun 1, 1997·Vaccine·J W ShiverM A Liu
Dec 14, 1999·Microbes and Infection·H L Davis, M J McCluskie
Apr 16, 2004·Clinical Microbiology Reviews·Malavika GiriDavid B Weiner
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·D R Burton
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·D H BarouchN L Letvin
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·N L LetvinJ W Shiver
Jan 31, 2007·Genetic Vaccines and Therapy·Gregers J GramJacob Glenting
Jan 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dan H BarouchNorman L Letvin
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S SantraN L Letvin

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