Humoral and T cell-mediated immune responses to bivalent killed bovine viral diarrhea virus vaccine in beef cattle

Veterinary Immunology and Immunopathology
Ratree PlattJames A Roth

Abstract

The objective of this research project was to evaluate the antibody and cell-mediated immune responses to a multivalent vaccine containing killed bovine viral diarrhea virus (BVDV) types 1 and 2. Twenty castrated male crossbred beef cattle (350-420kg body weight) seronegative to BVDV were randomly divided into two groups of 10 each. Group 1 served as negative mock-vaccinated control. Group 2 was vaccinated subcutaneously twice, 3 weeks apart, with modified live bovine herpesvirus 1, parainfluenza 3 virus and bovine respiratory syncytial virus diluted in diluent containing killed BVDV type 1 (strain 5960) and type 2 (strain 53637) in an adjuvant containing Quil A, Amphigen, and cholesterol. Serum samples were collected from all cattle at days -21, 0, and days 21, 28, 35, 56 and 70 post-vaccination. Standard serum virus neutralization tests were performed with BVDV type 1 (strain 5960) and type 2 (strain 125C). Anticoagulated blood samples were collected at day 0, and days 28, 35, 56 and 70 post-vaccination. Peripheral blood mononuclear cells (PBMCs) were isolated, stimulated with live BVDV type 1 (strain TGAN) and type 2 (strain 890) and cultured in vitro for 4 days. Supernatants of cultured cells were collected and saved for in...Continue Reading

References

Jul 1, 1995·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·H HoueJ W Lloyd
Oct 17, 2001·Journal of the American Veterinary Medical Association·M C ThurmondS K Hietala
Jan 10, 2003·American Journal of Veterinary Research·Julia E RidpathJames A Roth
May 29, 2003·Biologicals : Journal of the International Association of Biological Standardization·Janice J EndsleyJohn Neill

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Citations

Jun 15, 2011·Veterinary Immunology and Immunopathology·Ratree PlattJames A Roth
Feb 2, 2010·Veterinary Immunology and Immunopathology·Ratree PlattJames A Roth
Mar 9, 2018·Pathogens·Volker Moennig, Paul Becher
Oct 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuhiro NagaiTakahiro Yamaguchi
Jun 1, 2021·Frontiers in Veterinary Science·Anwar A G Al-KubatiMaged Gomaa Hemida

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