Humoral immune response to membrane components of Chlamydia trachomatis and expression of human 60 kDa heat shock protein in follicular fluid of in-vitro fertilization patients

Human Reproduction
A NeuerS S Witkin

Abstract

Recent evidence suggests that Chlamydia trachomatis can persist in the female upper genital tract in an unculturable state. Since unsuspected C. trachomatis infection has been associated with adverse in-vitro fertilization (IVF) outcome we sought to detect further evidence of C. trachomatis in the genital tracts of women undergoing IVF. The prevalence and distribution of antibodies to the major structural proteins of C. trachomatis in paired follicular fluid and sera of women undergoing IVF were examined. Sera and follicular fluid samples from 149 women were assayed for immunoglobulin (Ig)G and IgA antibodies to two C. trachomatis antigens, the major outer membrane protein (MOMP) and a recombinant lipopolysaccharide (rLPS) fragment. Additionally, the expression of human 60 kDa heat shock protein (hsp 60) in follicular fluid was determined. All cervical and follicular fluid samples were negative for C. trachomatis by polymerase chain reaction, ligase chain reaction and DNA probe. Sera from 60% of the subjects were positive for antichlamydial rLPS IgG; 36% were positive for anti-MOMP IgG. Similarly, rLPS-directed and MOMP-directed IgA were detected in sera of 34 and 14% of the subjects respectively. IgG antibodies to MOMP and rLP...Continue Reading

Citations

Aug 6, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Rajneesh JhaAruna Mittal
Oct 23, 2008·Journal of Assisted Reproduction and Genetics·Arthur BabayanSteven S Witkin
Oct 30, 2009·Clinical Reviews in Allergy & Immunology·Joan K Riley, D Michael Nelson
Mar 11, 2004·Archives of Medical Research·Paula CortiñasFlor Helene Pujol
Sep 12, 2009·Animal Reproduction Science·Melisa M L VelazquezHugo H Ortega
Mar 15, 2008·Fertility and Sterility·Arianna PacchiarottiCesare Aragona
Dec 14, 2007·American Journal of Reproductive Immunology : AJRI·Sharon JakusSteven S Witkin
Mar 3, 2018·Journal of Assisted Reproduction and Genetics·M G Da BroiP A Navarro
Jun 24, 2018·Scandinavian Journal of Immunology·Deepali Thaper, Vijay Prabha
Jul 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ozlem EquilsCalvin J Hobel
Aug 12, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Laila A IbrahimJohn J Bromfield
Jun 17, 1999·Infectious Diseases in Obstetrics and Gynecology·P C GiraldoS S Witkin
May 18, 2021·Frontiers in Cell and Developmental Biology·Babban JeeSubhradip Karmakar

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