Humoral immune responses against gut bacteria in dogs with inflammatory bowel disease

PloS One
Sirikul SoontararakSteven Dow

Abstract

Inflammatory bowel disease (IBD) in dogs is associated with clinical signs of intestinal dysfunction, as well as abnormal lymphocytic and myeloid cell infiltrates in the small and/or large intestine. Thus, in many respects IBD in dogs resembles IBD in humans. However, the factors that trigger intestinal inflammation in dogs with IBD are not well understood and have been variously attributed to immune responses against dietary antigens or intestinal antigens. Previous studies in humans with IBD have documented increased production of IgG and IgA antibodies specific to intestinal bacteria, and this abnormal immune response has been linked to disease pathogenesis. Therefore, we investigated the humoral immune response against gut bacteria in dogs with IBD, using flow cytometry to quantitate IgG and IgA binding. Studies were also done to investigate the source of these antibodies (locally produced versus systemic production) and whether greater antibody binding to bacteria is associated with increased inflammatory responses. We found that dogs with IBD had significantly higher percentages and overall amounts of IgG bound to their intestinal bacteria compared to healthy dogs. Similarly, significantly higher percentages of bacteria w...Continue Reading

References

Jan 1, 1977·International Archives of Allergy and Applied Immunology·D I GroveK S Warren
May 16, 2001·Science·L V Hooper, J I Gordon
May 31, 2003·Journal of Veterinary Internal Medicine·Albert E JergensRichard Evans
Jun 18, 2004·European Journal of Gastroenterology & Hepatology·Laurens A van der WaaijJohn O Hunter
May 4, 2006·Inflammatory Bowel Diseases·Sari IltanenMerja Ashorn
Jul 25, 2006·Infection and Immunity·Kenneth W SimpsonYnte H Schukken
Jul 27, 2007·Nature·R J Xavier, D K Podolsky
May 10, 2008·Journal of Veterinary Internal Medicine·I A BurgenerT W Jungi
Apr 11, 2009·PLoS Computational Biology·James Robert WhiteMihai Pop
Apr 15, 2010·Journal of Veterinary Internal Medicine·R J WashabauUNKNOWN WSAVA International Gastrointestinal Standardization Group
Aug 10, 2010·Current Opinion in Gastroenterology·Per Brandtzaeg
Apr 26, 2011·Gut·Anna HaasUNKNOWN Swiss HIV Cohort Study
Nov 19, 2011·Journal of Veterinary Internal Medicine·S L MarksJ S Weese
Feb 18, 2012·Clinical and Vaccine Immunology : CVI·Hermie J M HarmsenGerard Dijkstra
Aug 24, 2012·Lancet·Daniel C Baumgart, William J Sandborn
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
May 15, 2013·Journal of Veterinary Internal Medicine·D WalkerO A Garden
Feb 5, 2016·Veterinary and Comparative Oncology·G HartleyS Dow
Oct 4, 2016·Nature Microbiology·Yoshiki Vázquez-BaezaRob Knight
Feb 10, 2017·Gut·Victoria PascalChaysavanh Manichanh
Apr 7, 2018·Journal of Veterinary Internal Medicine·Cristiane C OtoniAlbert E Jergens

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
biopsy
ELISA
16S

Software Mentioned

Prism
stat
FlowJo
GraphPad
R
WGCNA
QIIME
Vegan
Uparse
Mothur

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.