Humoral immune responses by prime-boost heterologous route immunizations with CTB-MPR(649-684), a mucosal subunit HIV/AIDS vaccine candidate

Vaccine
Nobuyuki MatobaTsafrir S Mor

Abstract

CTB-MPR(649-684) is a translational fusion protein consisting of the cholera toxin B subunit and a 36-residue peptide, MPR(649-684), corresponding to the conserved membrane proximal ectodomain of gp41. CTB-MPR(649-684) was previously shown to induce HIV-1 transcytosis-blocking antibodies in mice. In this report, we describe an effective immunization regimen for this novel anti HIV-1 vaccine-candidate. Bacterially-produced CTB-MPR(649-684) was intranasally and/or intraperitoneally administered to investigate several prime-boost heterologous route immunization regimens. Mucosal priming with the adjuvant cholera toxin elicited significant levels of vaginal IgA and serum IgG specific to MPR(649-684). Systemic boosting after mucosal priming enhanced the levels of serum and mucosal antibodies. Systemic priming induced a strong serum anti-MPR(649-684) IgG response, which was efficiently recalled and augmented by either systemic or mucosal boosting. However, this regimen was less effective in inducing secretory anti-MPR(649-684) IgA. The serum anti-MPR(649-684) IgG subtype profile revealed that both IgG1 and IgG2a were induced in all the immunization regimens, and that mucosal co-administration of cholera toxin shifted the bias to the ...Continue Reading

References

Feb 1, 2002·Nature Medicine·Gang MengPhillip D Smith
Jun 29, 2002·Current Opinion in Immunology·Flavia Ferrantelli, Ruth M Ruprecht
Jul 26, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Claudia DevitoKristina Broliden
Apr 18, 2003·Current Molecular Medicine·Pamela A Kozlowski, Marian R Neutra
Jul 2, 2003·Nature Medicine·Melissa Pope, Ashley T Haase
Aug 22, 2003·Reviews in Medical Virology·Yoshikazu Yuki, Hiroshi Kiyono
Mar 25, 2004·Nature Reviews. Immunology·Susan Zolla-Pazner
Apr 30, 2004·Nature Immunology·Myron M Levine, Marcelo B Sztein
Aug 3, 2004·Expert Review of Vaccines·Indresh K SrivastavaSusan W Barnett
Aug 3, 2004·Expert Review of Vaccines·Igor M BelyakovJay A Berzofsky
Sep 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Nobuyuki MatobaTsafrir S Mor
Sep 15, 2004·The Journal of Experimental Medicine·Jason M BrenchleyDaniel C Douek
Sep 24, 2004·Trends in Microbiology·Annemarie N LekkerkerkerTeunis B H Geijtenbeek
Jan 26, 2005·AIDS·Donald P Kotler

❮ Previous
Next ❯

Citations

Sep 9, 2010·Cancer Immunology, Immunotherapy : CII·Julia PinkhasovPinku Mukherjee
Sep 1, 2007·Current Opinion in HIV and AIDS·Heather B Jaspan, Willem A Hanekom
Jul 14, 2010·Expert Opinion on Biological Therapy·Mingke Yu, Michael Vajdy
Dec 14, 2011·Journal of Reproductive Immunology·Rajesh K Naz
Mar 10, 2009·Molecular Immunology·Shenghua LiJianbing Zhang
Jun 14, 2008·Analytical Biochemistry·Katie A EdwardsJohn C March
Sep 23, 2011·Veterinary Ophthalmology·Elizabeth A Giuliano, Kevin Finn
Aug 27, 2014·Protein Science : a Publication of the Protein Society·Zhen GongPetra Fromme
Mar 25, 2015·Toxins·Keegan J BaldaufNobuyuki Matoba
Sep 9, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jérôme CoutantMorgane Bomsel
Aug 28, 2014·The Journal of Biological Chemistry·Rachel P J LaiWinfried Weissenhorn
Jan 6, 2011·The Journal of Clinical Investigation·Bangxing HongSi-Yi Chen
Dec 10, 2008·International Reviews of Immunology·Marc P Girard, Geetha P Bansal

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.