Humoral immunity in mice mediated by monoclonal antibodies against the A and M antigens of Brucella

Journal of Medical Microbiology
J N LimetM Plommet

Abstract

All smooth strains of Brucella bear two lipopolysaccharide (LPS) antigens in a ratio that defines the classification of strains in serovars, A (A greater than M), M (M greater than A) and A.M (A = M). Anti-LPS-A monoclonal antibodies (MAb-A) were previously shown to convey protection to mice against B. abortus (A) strain 544, as shown by lower spleen counts than in controls at days 7 and 21 after challenge. Anti-LPS-M monoclonal antibodies (MAb-M) were obtained and tested for M-specificity with LPS from reference strains by ELISA, by agglutination of LPS-coated latex particles, and by inhibition of this agglutination. Antigens A and M of three strains were quantified by a homologous LPS-latex and MAb agglutination inhibition assay. Protection conferred by MAb-A and MAb-M against three strains, B. abortus 544 (A), B. abortus 292 (M) and B. melitensis H38 (M), was tested at equivalent challenge and MAb doses: intravenous challenge was adjusted to give similar infection at day 7; MAb doses were adjusted to the same specific ELISA titre. Under these conditions, MAb-A and MAb-M conferred both early and late protection, as shown at days 7 and 21, against the strains that bore the homologous major antigen, i.e., strain 544 on one hand...Continue Reading

Citations

May 7, 2004·Molecular Immunology·Thierry Cl LaurentXavier De Bolle
Nov 5, 2002·Veterinary Microbiology·Axel CloeckaertPhilip H Elzer
Aug 6, 2002·Research in Microbiology·Sylvie BaucheronAxel Cloeckaert
Nov 16, 2001·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·N RojasE Moreno
Jul 5, 2012·Clinical and Vaccine Immunology : CVI·Michel S ZygmuntAxel Cloeckaert
Mar 12, 2013·Case Reports in Ophthalmological Medicine·Claudia BruèAlfonso Giovannini
Apr 17, 2012·Veterinary Research·María-Jesús GrillóEdgardo Moreno
Jul 28, 2009·Peptides·Concetta BeninatiFranco Felici
Nov 11, 1999·Journal of Molecular Biology·X De BolleP Mertens

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