PMID: 1194850Nov 1, 1975Paper

Humoral immunostimulation. V. Selection of variant cell lines

The Journal of Experimental Medicine
W T Shearer, C W Parker

Abstract

A permanent L-cell variant cell line (LC1) was isolated by the growth of the parent L-cell line (L) in the presence of a cytostimulatory dose (1:200) of rabbit anti-L-cell antiserum (AL) for 9 mo. LC1 differed from L in many aspects: (a) it was larger (1,533 mm3 vs. 1,284 mm3), (b) it grew faster (1.5- to 2-fold), (c) it grew in aggregated fashion, (d) its growth was no longer stimulated by AL, (e) it was almost completely resistant to high concentrations of AL in the presence of complement (C), (f) its original membrane antigens (immunogenic for AL) were redistributed in sparse and patchy clumps as noted by fluorescence microscopy, (g) it contained about 65% of the total original 125I-AL membrane-binding sites (1.4 X 10(7)/cell vs. 2.2 X 10(7)/cell), (h) its AL-binding sites displayed a lower average affinity constant (K = 0.9 X 10(5) M-1 vs. 2.8 X 10(5) M-1), (i) it contained a smaller proportion of high affinity (K greater than 10(6) M-1) binding sites (13% vs 21%), and (j) LC1 was fully immunogenic in that it was readily killed by homologous antiserum (ALC1) and C, whereas L was not similarly affected by ALC1 indicating that LC1 contained new membrane antigens not present on L. Another variant (LC2) was produced by growth o...Continue Reading

References

May 29, 1975·Nature·M P FinkW T Shearer
Dec 28, 1973·Science·W T ShearerC W Parker
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·T T PuckF T Kao
Oct 15, 1974·International Journal of Cancer. Journal International Du Cancer·W T ShearerC W Parker
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C GottliebS Kornfeld
Oct 7, 1958·Annals of the New York Academy of Sciences·P A GORER
Aug 1, 1960·Archives of Biochemistry and Biophysics·A NISONOFFD L WOERNLEY

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