Humoral response against sialyl-Le(a) glycosylated protein species in esophageal cancer: Insights for immunoproteomic studies

Electrophoresis
Elisabete FernandesJosé Alexandre Ferreira

Abstract

Esophageal cancers (ECs) show poor prognosis and decreased overall survival due to late diagnosis and ineffective therapeutics, urging the introduction of novel biomarkers to aid disease management. The levels of sialyl-Lewis(a) antigen (sLe(a) ) are frequently increased in digestive tumours, which has been explored in serological non-invasive prognostication (CA19-9 test); however, with low sensitivity and specificity. Autoantibodies against cancer antigens are considered the next generation biomarkers, as they are present in circulation long before tumour-associated proteins. Based on these observations we have mined the serum of EC patients (n = 7) for antibodies against sLe(a) -glycosylated protein species. All EC were positive for sLe(a) , irrespectively of their histological nature but only two patients showed elevated CA19-9. Moreover, IgG titers, with emphasis on IgG1, were elevated in EC patients in comparison to the control group. SLe(a) -glycoproteins were then extracted from tumours of patients with negative CA19-9, isolated by immunoprecipitation and blotted with patients IgG. Autoantibodies against sLe(a) -glycosylated proteins were detected in all cases. Different SLe(a) -glycoproteins were observed for tumours o...Continue Reading

References

Aug 1, 1989·British Journal of Cancer·A McKnightI Shperling
Oct 1, 1996·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·K MealyT P Hennessy
Dec 2, 1999·Biochimica Et Biophysica Acta·I Brockhausen
Sep 8, 2001·Journal of Mammary Gland Biology and Neoplasia·J M BurchellJ Taylor-Papadimitriou
Dec 29, 2004·Surgery Today·Hiroyuki KuwanoAhmad Faried
Aug 9, 2005·Journal of Proteome Research·Karen S Anderson, Joshua LaBaer
Feb 24, 2011·International Journal of Cancer. Journal International Du Cancer·Johannes W PedersenHans H Wandall
Jun 10, 2011·Journal of Cellular and Molecular Medicine·C DesmetzJ Solassol
Aug 9, 2011·Expert Review of Proteomics·Celso A ReisLúcio Lara Santos
Sep 3, 2011·Journal of Cellular and Molecular Medicine·Rita PintoLeonor David
Aug 14, 2012·International Journal of Cancer. Journal International Du Cancer·Jérôme LacombeJérôme Solassol
Jan 22, 2013·The International Journal of Biochemistry & Cell Biology·Lydia MareMarco Trinchera
Oct 15, 2013·International Journal of Cancer. Journal International Du Cancer·Johannes W PedersenHans H Wandall
Mar 14, 2014·Expert Review of Anticancer Therapy·Andrew CowieTimothy Underwood
Dec 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hongfei ZhangJianying Zhang

❮ Previous
Next ❯

Citations

Nov 2, 2019·Expert Review of Molecular Diagnostics·Anna BlsakovaJan Tkac
Apr 13, 2021·Frontiers in Immunology·Ming CuiXiaoyan Qiu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Annals of Oncology : Official Journal of the European Society for Medical Oncology
C ChapmanJ Robertson
Rinshō Kyōbu Geka = Japanese Annals of Thoracic Surgery
R KannagiK Hashimoto
© 2021 Meta ULC. All rights reserved