HupO, a Novel Regulator Involved in Thiosulfate-Responsive Control of HupSL [NiFe]-Hydrogenase Synthesis in Thiocapsa roseopersicina

Applied and Environmental Microbiology
Ildikó K NagyGergely Maróti

Abstract

[NiFe]-hydrogenases are regulated by various factors to fulfill their physiological functions in bacterial cells. The photosynthetic purple sulfur bacterium Thiocapsa roseopersicina harbors four functional [NiFe]-hydrogenases: HynSL, HupSL, Hox1, and Hox2. Most of these hydrogenases are functionally linked to sulfur metabolism, and thiosulfate has a central role in this organism. The membrane-associated Hup hydrogenases have been shown to play a role in energy conservation through hydrogen recycling. The expression of Hup-type hydrogenases is regulated by H2 in Rhodobacter capsulatus and Cupriavidus necator; however, it has been shown that the corresponding hydrogen-sensing system is nonfunctional in T. roseopersicina and that thiosulfate is a regulating factor of hup expression. Here, we describe the discovery and analysis of mutants of a putative regulator (HupO) of the Hup hydrogenase in T. roseopersicina. HupO appears to mediate the transcriptional repression of Hup enzyme synthesis under low-thiosulfate conditions. We also demonstrate that the presence of the Hox1 hydrogenase strongly influences Hup enzyme synthesis in that hup expression was decreased significantly in the hox1 mutant. This reduction in Hup synthesis could...Continue Reading

References

Jul 1, 1983·Annales de microbiologie·N Pfennig, H G Trüper
Dec 1, 1996·Analytical Biochemistry·G Rakhely, K L Kovacs
Aug 29, 2001·FEMS Microbiology Reviews·P M VignaisJ Meyer
Jan 5, 2002·Applied and Environmental Microbiology·Rikard Axelsson, Peter Lindblad
Feb 10, 2004·Applied and Environmental Microbiology·Barna D FodorKornél L Kovács
Apr 5, 2005·Journal of Bacteriology·Akos T KovácsKornél L Kovács
Sep 15, 2005·The FEBS Journal·Akos T KovácsKornél L Kovács
Nov 10, 2006·Advances in Microbial Physiology·August BöckMatthew C Posewitz
Mar 23, 2007·Biochimica Et Biophysica Acta·Gábor RákhelyKornél L Kovács
Oct 2, 2007·FEMS Microbiology Reviews·Paula TamagniniPeter Lindblad
Oct 12, 2007·Chemical Reviews·Paulette M Vignais, Bernard Billoud
Nov 20, 2008·The Journal of Biological Chemistry·Marcus LudwigBärbel Friedrich
Nov 21, 2008·The FEBS Journal·Lívia S Palágyi-MészárosKornél L Kovács
Jun 15, 2010·Applied and Environmental Microbiology·Judit MarótiKornél L Kovács

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Citations

Jan 16, 2020·International Journal of Molecular Sciences·Azat Vadimovich Abdullatypov

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