Abstract
Lung cancer is the most frequent cancer type and the leading cause of tumor-associated deaths worldwide. TP53 is an important tumor suppressor gene and is frequently inactivated in lung cancer. E3 ligases targeting p53, such as MDM2, are involved in the development of lung cancer. The E3 ligase HUWE1, which targets many tumor-associated proteins including p53, has been reported to be highly expressed in lung cancer; however, its role in lung tumorigenesis is unclear. Methods: The expression of HUWE1 and p53 in lung cancer cells was modulated and the phenotypes were assessed by performing soft agar colony forming assays, cell cycle analysis, BrdU incorporation assays, and xenograft tumor growth assays. The effect on tumorigenesis in genetically-engineered mice was also analyzed. The mechanism through which HUWE1 sustained lung cancer cell malignancy was confirmed by western blotting. HUWE1 expression in clinical lung cancer was identified by immunohistochemistry and validated by analyzing lung adenocarcinoma and lung squamous carcinoma samples from the Cancer Genome Atlas (TCGA) database. Finally, we assessed the association between HUWE1 expression and patient outcome using online survival analysis software including survival i...Continue Reading
Citations
Aug 9, 2018·Chembiochem : a European Journal of Chemical Biology·Dan ChenSonja Lorenz
May 28, 2019·Cells·Monica Vara-PerezPatrizia Agostinis
Jul 11, 2019·Journal for Immunotherapy of Cancer·Dong YangXudong Zhao
Jun 12, 2020·Oncogene·Lisa J CrawfordAlexandra E Irvine
Jul 11, 2019·Neural Regeneration Research·Guo-Qian HeWei Zhang
May 16, 2020·PeerJ·Xiaohan MaLiang Ming
Sep 29, 2020·Journal of Translational Internal Medicine·Hao QianYingxian Sun
Sep 5, 2020·International Journal of Molecular Sciences·Gizem CelebiOzlem Kutlu
Feb 3, 2020·Investigational New Drugs·Xiaofeng GongShengtao Yuan
Oct 27, 2020·The Journal of Clinical Investigation·Yu GuoXiaopeng Qi
Nov 18, 2020·Biochemical Society Transactions·Joe MaltasAngeliki Malliri
Jan 7, 2021·Investigational New Drugs·Yuting HeQinsi Wan
Jul 8, 2019·Gene·Yuanyuan ZhangHaiyan Xu
Jan 10, 2021·Biochemical Pharmacology·Zhifei XuPeihua Luo
Jun 3, 2021·Cells·Stijn WenmaekersRichard P Meijer