HYAL1 and HYAL2 inhibit tumour growth in vivo but not in vitro.

PloS One
Fuli WangEugene Zabarovsky

Abstract

We identified two 3p21.3 regions (LUCA and AP20) as most frequently affected in lung, breast and other carcinomas and reported their fine physical and gene maps. It is becoming increasingly clear that each of these two regions contains several TSGs. Until now TSGs which were isolated from AP20 and LUCA regions (e.g.G21/NPRL2, RASSF1A, RASSF1C, SEMA3B, SEMA3F, RBSP3) were shown to inhibit tumour cell growth both in vitro and in vivo. The effect of expression HYAL1 and HYAL2 was studied by colony formation inhibition, growth curve and cell proliferation tests in vitro and tumour growth assay in vivo. Very modest growth inhibition was detected in vitro in U2020 lung and KRC/Y renal carcinoma cell lines. In the in vivo experiment stably transfected KRC/Y cells expressing HYAL1 or HYAL2 were inoculated into SCID mice (10 and 12 mice respectively). Tumours grew in eight mice inoculated with HYAL1. Ectopic HYAL1 was deleted in all of them. HYAL2 was inoculated into 12 mice and only four tumours were obtained. In 3 of them the gene was deleted. In one tumour it was present but not expressed. As expected for tumour suppressor genes HYAL1 and HYAL2 were down-expressed in 15 fresh lung squamous cell carcinomas (100%) and clear cell RCC tu...Continue Reading

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Citations

Dec 24, 2013·Acta Biomaterialia·Kevin T DickerXinqiao Jia
Oct 18, 2014·Cancer Metastasis Reviews·Anja SchmausJonathan P Sleeman
Jan 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·George Klein
Sep 29, 2009·The Journal of Biological Chemistry·Cecile DutermeBruno Flamion
Sep 21, 2010·BMC Cancer·Helen BougaDemitrios H Vynios
Dec 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Francesco AcquatiRoberto Taramelli
Jun 4, 2011·Molekuliarnaia biologiia·G S KrasnovV N Senchenko
Jun 27, 2015·Experimental and Molecular Pathology·Melanie MaierthalerBarbara Burwinkel
Sep 13, 2014·International Journal of Cancer. Journal International Du Cancer·Rongxi YangBarbara Burwinkel
May 18, 2010·International Journal of Cancer. Journal International Du Cancer·Jin-Xiang TanGuo-Sheng Ren
Aug 13, 2013·Gene·A A StepanenkoV M Kavsan
Oct 12, 2012·PloS One·Aleksandr G KondratovVladimir I Kashuba
Oct 18, 2016·Pancreas·Shiro KohiKeiji Hirata

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Methods Mentioned

BETA
PCR
transfection
Assay
biopsies
surgical resection
electrophoresis

Software Mentioned

CyQUANT
BioStat

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