PMID: 8941769Jun 1, 1996Paper

Hyaluronan, a possible ligand mediating Treponema denticola binding to periodontal tissue

Oral Microbiology and Immunology
M HaapasaloV J Uitto

Abstract

Binding of Treponema denticola ATCC 35405 to glycosaminoglycans, fibrinogen, type I collagen and porcine periodontal ligament epithelial cells was studied using an enzyme-linked immunosorbent assay. T. denticola bound to hyaluronan (hyaluronic acid) and its hexameric fragments. whereas little or no binding was detected to chondroitin-4-sulfate or dermatan sulfate proteoglycan. Binding of T. denticola to hyaluronan gradually increased during the 2-h incubation time. In contrast, binding to fibrinogen and type I collagen was more rapid, peaking within 5 min. T. denticola also bound to microbeads coated with hyaluronan and formed visible aggregates in solution. Pretreatment of the bacteria with hyaluronan or fibrinogen inhibited binding to hyaluronan. Gelatin, bovine serum albumin, chondroitin-4-sulfate, chondroitin-6-sulfate, heparin, dermatan sulfate, glucuronic acid, N-acetylglucosamine and N-acetyl-galactosamine did not inhibit binding. Binding was also inhibited by heating T. denticola and by pretreatment of the spirochetes with sodium periodate, phenylmethylsulfonyl fluoride, and p-chloromercurybenzoic acid. All these treatments also inhibited the chymotrypsin-like activity of T. denticola. Hyaluronan strongly inhibited bind...Continue Reading

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Citations

Jan 13, 2001·Oral Microbiology and Immunology·E C Chan, R McLaughlin
Jan 1, 1997·Microbiology and Immunology·T UmemotoI Namikawa
Mar 1, 2012·Journal of Oral Microbiology·J Christopher Fenno
Apr 27, 2004·Periodontology 2000·Neil M O'Brien-SimpsonEric C Reynolds
Aug 5, 2006·Anaerobe·J C Fenno, B C McBride
Mar 19, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M B Visser, R P Ellen
Mar 27, 2003·International Endodontic Journal·I N RôçasM Uzeda
Aug 3, 2001·International Endodontic Journal·J F SiqueiraK R Santos
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M N Sela
Apr 1, 1997·Indian Journal of Psychiatry·D R Doongaji
Jun 20, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Andressa Temperine de Oliveira MarreLeandro A Lobo
Mar 30, 2021·Journal of Animal Science·Patrícia M ObaKelly S Swanson

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