Hyaluronan antagonizes the differentiation effect of TGF-β1 on nasal epithelial cells through down-regulation of TGF-β type I receptor

Artificial Cells, Nanomedicine, and Biotechnology
Tsung-Wei HuangTai-Horng Young

Abstract

Although hyaluronan (HA)-based biomaterials have been proposed to promote mucociliary differentiation of nasal epithelial cells (NECs), the mechanism by which HA affects the growth and differentiation of NECs has not been thoroughly explored. This study investigates the effect and mechanism of HA on the differentiation of NECs. The experiment cultures human NECs in four conditions, namely controls, transforming growth factor (TGF)-β1, TGF-β1 + HA and HA groups. In the TGF group, the NECs become irregular shape without formation of tight junction and mucociliary differentiation of NECs is inhibited. Epithelial-mesenchymal transition (EMT) of NECs also occurs in the TGF group. However, with addition of HA in TGF groups, NECs reveal the mucociliary phenotypes of epithelial cells with tight junction expression. Incubation of TGF-β1 in an NEC culture leads to an increase in phosphorylated type 1 TGF-β receptors (p-TβRI). This increase is attenuated when NECs are cultured in the presence of HA. Similar expressions are observed in phosphorylated smad2/smad3. Additionally, HA-dependent inhibition of TGF-β1 signalling is inhibited by co-incubation with a blocking antibody to CD44. Experimental results indicate that HA can antagonize TGF...Continue Reading

References

Nov 10, 1994·The New England Journal of Medicine·W A Border, N A Noble
Aug 4, 1994·Nature·J L WranaJ Massagué
Dec 1, 1996·Cytokine & Growth Factor Reviews·L Attisano, J L Wrana
Dec 5, 1997·The European Respiratory Journal·R WuM M Chang
May 23, 1998·Annual Review of Immunology·J J Letterio, A B Roberts
Oct 6, 1998·Annual Review of Biochemistry·J Massagué
Jan 23, 1999·The Laryngoscope·H H Ramadan
Feb 26, 1999·The Journal of Cell Biology·S BanerjiD G Jackson
Jun 3, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·D J ThorntonJ K Sheehan
Oct 20, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R FortezaM Salathe
Dec 14, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·William J HowatPeter M Lackie
Aug 20, 2003·Journal of Biomedical Materials Research. Part a·L HuangS M Kumta
Oct 17, 2006·The Annals of Otology, Rhinology & Laryngology. Supplement·Noam A Cohen
May 31, 2007·The British Journal of Dermatology·T WongH Navsaria
Jul 17, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Brigham C Willis, Zea Borok
Jan 21, 2011·Physiological Reviews·Dianhua JiangPaul W Noble
Nov 19, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·N Van BruaeneC Bachert
Jan 15, 2015·Journal of Laboratory Automation·Balaji SrinivasanJames J Hickman
Aug 13, 2015·American Journal of Rhinology & Allergy·Mingjie WangBing Zhou
Dec 8, 2015·American Journal of Rhinology & Allergy·Kai-Min FangTsung-Wei Huang

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