Abstract
CD44 is an adhesion receptor for which the major characterized ligand is the extracellular matrix glycosaminoglycan, hyaluronan. This interaction underlies CD44-mediated cell attachment, cell migration, and matrix remodelling during development and wound healing. Truncation of the CD44 cytoplasmic domain does not prevent cell surface expression of this hyaluronan receptor but it dramatically impairs ligand binding. In this study we have examined the role of phosphorylation in regulating this function by mutating the target serine residues to either neutral amino acids with the aim of creating a phosphorylation-incompetent molecule, or to acidic residues to mimic a fully phosphorylated CD44. In transfected AKR1 cells the behavior of both the neutral and acidic mutants was indistinguishable from wild-type CD44, indicating that there is a phosphorylation-independent mechanism involved in regulating hyaluronan binding.
References
Feb 1, 1992·The Journal of Cell Biology·M CultyC B Underhill
Aug 1, 1992·The Journal of Cell Biology·L ThomasI Stamenkovic
Dec 1, 1992·The Journal of Cell Biology·Q HeP W Kincade
Jun 29, 1990·Cell·A AruffoB Seed
Dec 1, 1990·The Journal of Cell Biology·M CultyE Silorski
Jan 1, 1992·The Journal of Experimental Medicine·J LesleyP W Kincade
Dec 1, 1991·The Journal of Cell Biology·R L CampE Puré
Jun 1, 1990·The Journal of Cell Biology·L M ShawA M Mercurio
Apr 1, 1990·Experimental Cell Research·J LesleyR Hyman
Mar 24, 1989·Cell·I StamenkovicB Seed
Jan 1, 1986·Immunogenetics·C M IsackeI S Trowbridge
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Mar 1, 1982·Immunogenetics·I S TrowbridgeJ Trotter
Jul 1, 1994·The Journal of Cell Biology·S TsukitaS Tsukita
Aug 1, 1994·The Journal of Cell Biology·V B LokeshwarL Y Bourguignon
Jan 1, 1995·The Journal of Experimental Medicine·E PuréS Nayak
Oct 1, 1994·Current Opinion in Cell Biology·L ShermanH Ponta
Aug 1, 1993·European Journal of Immunology·J LesleyR Hyman
Aug 1, 1993·Immunology Today·P HerrlichH Ponta
Jun 17, 1994·Cell·S CowleyC J Marshall
Jul 1, 1993·The Journal of Cell Biology·R J PeachA Aruffo
Jan 1, 1993·Advances in Immunology·J LesleyP W Kincade
Jun 1, 1993·The Journal of Cell Biology·S J Neame, C M Isacke
Citations
Feb 12, 1998·Cancer Letters·B S KubensK S Zänker
Oct 23, 1997·Current Opinion in Cell Biology·P W KincadeL Hanson
Jul 21, 2001·The Biochemical Journal·C A LewisC M Isacke
Feb 4, 2011·Molecular Biology of the Cell·J L CannonA I Sperling
May 7, 2011·Current Opinion in Hematology·Robert Sackstein
Sep 3, 1998·The Journal of Clinical Investigation·J MollP Herrlich
Jul 31, 2008·Blood·Rahima ZennadiMarilyn J Telen
Mar 14, 2000·Cell Adhesion and Communication·M RochmanD Naor
Feb 4, 1999·Cell Adhesion and Communication·R V Sionov, D Naor
Nov 21, 1998·Cell Adhesion and Communication·H SheikhC Isacke
Aug 12, 1998·The Journal of Investigative Dermatology·J M WeissJ C Simon
Aug 15, 2014·Proteins·Christina E Faller, Olgun Guvench
Jul 3, 2015·Frontiers in Immunology·Olgun Guvench
Jul 1, 1996·Current Biology : CB·D Peck, C M Isacke
Jun 25, 1998·Current Biology : CB·J W Legg, C M Isacke
Jan 17, 2002·The Journal of Biological Chemistry·Hong JiangWarren Knudson
May 24, 1996·The Journal of Biological Chemistry·H Sheikh, C M Isacke
Jun 21, 2006·Oncogene·G TzircotisC M Isacke
Jun 9, 1998·Immunology·G BorlandK Guy
Jan 14, 2005·The Journal of Biological Chemistry·Edward R BastowAndrew A Pitsillides
Oct 20, 2005·Journal of Cell Science·George TzircotisClare M Isacke
May 1, 1999·Cellular Immunology·M C Levesque, B F Haynes
Sep 4, 2021·The FEBS Journal·Qian GuoFeng Gao