Hyaluronan binding by CD44 is regulated by a phosphorylation-independent mechanism

European Journal of Immunology
C R UffC M Isacke

Abstract

CD44 is an adhesion receptor for which the major characterized ligand is the extracellular matrix glycosaminoglycan, hyaluronan. This interaction underlies CD44-mediated cell attachment, cell migration, and matrix remodelling during development and wound healing. Truncation of the CD44 cytoplasmic domain does not prevent cell surface expression of this hyaluronan receptor but it dramatically impairs ligand binding. In this study we have examined the role of phosphorylation in regulating this function by mutating the target serine residues to either neutral amino acids with the aim of creating a phosphorylation-incompetent molecule, or to acidic residues to mimic a fully phosphorylated CD44. In transfected AKR1 cells the behavior of both the neutral and acidic mutants was indistinguishable from wild-type CD44, indicating that there is a phosphorylation-independent mechanism involved in regulating hyaluronan binding.

References

Feb 1, 1992·The Journal of Cell Biology·M CultyC B Underhill
Aug 1, 1992·The Journal of Cell Biology·L ThomasI Stamenkovic
Jan 1, 1986·Immunogenetics·C M IsackeI S Trowbridge
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Jul 1, 1993·The Journal of Cell Biology·R J PeachA Aruffo
Jan 1, 1993·Advances in Immunology·J LesleyP W Kincade

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Citations

Oct 23, 1997·Current Opinion in Cell Biology·P W KincadeL Hanson
Sep 3, 1998·The Journal of Clinical Investigation·J MollP Herrlich
Feb 4, 1999·Cell Adhesion and Communication·R V Sionov, D Naor
Jun 9, 1998·Immunology·G BorlandK Guy

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