Hyaluronan concentration and size distribution in human knee synovial fluid: variations with age and cartilage degeneration

Arthritis Research & Therapy
Michele M Temple-WongRobert L Sah

Abstract

One potential mechanism for early superficial cartilage wear in normal joints is alteration of the lubricant content and quality of synovial fluid. The purpose of this study was to determine if the concentration and quality of the lubricant, hyaluronan, in synovial fluid: (1) was similar in left and right knees; (2) exhibited similar age-associated trends, whether collected postmortem or antemortem; and (3) varied with age and grade of joint degeneration. Human synovial fluid of donors (23-91 years) without osteoarthritis was analyzed for the concentrations of protein, hyaluronan, and hyaluronan in the molecular weight ranges of 2.5-7 MDa, 1-2.5 MDa, 0.5-1 MDa, and 0.03-0.5 MDa. Similarity of data between left and right knees was assessed by reduced major axis regression, paired t-test, and Bland-Altman analysis. The effect of antemortem versus postmortem collection on biochemical properties was assessed for age-matched samples by unpaired t-test. The relationships between age, joint grade, and each biochemical component were assessed by regression analysis. Joint grade and the concentrations of protein, hyaluronan, and hyaluronan in the molecular weight ranges of 2.5-7 MDa, 1-2.5 MDa, and 0.5-1 MDa in human synovial fluid show...Continue Reading

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Citations

Sep 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jacek SiódmiakAdam Gadomski
Mar 27, 2018·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·Carla SteccoRaffaele De Caro
Oct 27, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Edward D BonnevieLawrence J Bonassar
Nov 8, 2018·Journal of Tissue Engineering and Regenerative Medicine·Ahmad E Abu-HakmehLeo Q Wan
Feb 7, 2019·The FEBS Journal·Anastasia G TavianatouNikos K Karamanos
Jan 21, 2020·Journal of Biomechanical Engineering·Edward D Bonnevie, Lawrence J Bonassar
Mar 14, 2020·International Journal of Molecular Sciences·Tao ChenAndreas K Nussler
Apr 11, 2019·Polymers·Tomasz AndrysiakDamian Ledziński
Jul 7, 2019·Rheumatic Diseases Clinics of North America·Carrie RichardsonJoel A Block
Feb 16, 2021·Macromolecules·Giulia GiubertoniHuib J Bakker
May 8, 2018·Journal of Biomechanics·Edward D BonnevieLawrence J Bonassar
Apr 7, 2021·Critical Reviews in Biochemistry and Molecular Biology·Shuhui ChenLara K Mahal
Jun 8, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Maurice C E van DalenMireille M A E Claessens

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Methods Mentioned

BETA
electrophoresis

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