Hyaluronan microenvironment enhances cartilage regeneration of human adipose-derived stem cells in a chondral defect model

International Journal of Biological Macromolecules
Shun-Cheng WuMei-Ling Ho

Abstract

Hyaluronan (HA) is an important extracellular matrix component in the early stage of chondrogenesis. This study aimed to investigate the application of an HA microenvironment for human adipose-derived stem cells (hADSCs)-based articular cartilage regeneration. HA-enriched fibrin (HA/Fibrin) hydrogels were synthesized and characterized for use as HA microenvironments. The cell viability and chondrogenic gene expression of hADSCs cultured in HA/Fibrin (HA/Fibrin/hADSC) and Fibrin (Fibrin/hADSC) hydrogels were tested in vitro. A chondral defect created in osteochondral core explants ex vivo was used to test chondral defect regeneration by HA/Fibrin/hADSC or Fibrin/hADSC hydrogels. The results showed that HA/Fibrin hydrogels exhibited an increased swelling ratio and matrix stiffness and a smoother surface with more interconnected pores than in Fibrin hydrogels. The viability of hADSCs in HA/Fibrin/hADSC hydrogels was not altered, but they exhibited higher chondrogenic gene expression than those in Fibrin/hADSC hydrogels. For chondral defect regeneration, the HA/Fibrin/hADSC hydrogels exhibited the most cartilaginous tissue neo-formation, chondral integration and sGAG content in the surrounding tissue. This study demonstrated that a...Continue Reading

References

Feb 1, 1992·The Journal of Cell Biology·M CultyC B Underhill
Dec 1, 1985·Developmental Biology·C B Knudson, B P Toole
Mar 1, 1973·Annals of the Rheumatic Diseases·K N AntonasK D Muirden
Jan 1, 1995·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·F H SilverG D Pins
Apr 1, 1996·Emerging Infectious Diseases·K C ClarkeB J Tempalski
Aug 15, 1998·Arthritis and Rheumatism·J A Buckwalter, H J Mankin
Dec 16, 1998·The American Journal of Sports Medicine·B R MandelbaumL Peterson
May 12, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T M QuinnE B Hunziker
Sep 27, 2001·Journal of Cellular Physiology·S GronthosJ M Gimble
Jan 10, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B KurzA J Grodzinsky
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 26, 2002·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Mark R HomiczDeborah Watson
Feb 8, 2003·Human Gene Therapy·Kouki MorizonoMarc H Hedrick
Sep 6, 2003·Birth Defects Research. Part C, Embryo Today : Reviews·Cheryl B Knudson
Mar 17, 2004·Arthritis and Rheumatism·Michael A DiMiccoAlan J Grodzinsky
Dec 8, 2004·Stem Cells·Boon Chin HengEng Hin Lee
Dec 14, 2004·Tissue Engineering·Timothy S JohnsonMichael J Yaremchuk
Jan 27, 2005·Cell and Tissue Research·Jinwu ChenDexue Li
Jun 3, 2005·Clinical Orthopaedics and Related Research·Maurilio MarcacciStefano Zanasi
Jul 29, 2005·The Journal of Bone and Joint Surgery. British Volume·M AkmalG Bentley
Dec 3, 2005·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Bodo KurzMichael Schünke
Mar 14, 2007·Stem Cells and Development·Maikel J Oedayrajsingh VarmaFlorine J van Milligen
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Muzlifah A HaniffaMatthew P Collin
May 28, 2008·Injury·Karoliina PelttariWiltrud Richter
Oct 25, 2008·Arthritis Research & Therapy·Faye H Chen, Rocky S Tuan
Dec 11, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yan-Hsiung WangGwo-Jaw Wang
Sep 22, 2009·Journal of Internal Medicine·W Richter
Feb 27, 2010·Journal of Biomedical Materials Research. Part a·J Tyler EndersZijun Zhang
Jan 1, 2009·Critical Reviews in Biomedical Engineering·Lijie ZhangKyriacos A Athanasiou

❮ Previous
Next ❯

Citations

Jan 27, 2019·International Journal of Molecular Sciences·Pei-Lin ShaoChau-Zen Wang
Jan 19, 2019·Current Pharmaceutical Design·Chahinez HouacineKamalinder K Singh
Mar 7, 2020·Genetics and Molecular Biology·Maria Inês WitsNance Beyer Nardi
Jul 6, 2019·Current Medicinal Chemistry·Shuaimeng GuanJingan Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Attention Disorders

Attention is involved in all cognitive activities, and attention disorders are reported in patients with various neurological diseases. Here are the latest discoveries pertaining to attention disorders.