Hyaluronan mixed esters of butyric and retinoic acid affording myocardial survival and repair without stem cell transplantation.

The Journal of Biological Chemistry
Vincenzo LionettiCarlo Ventura

Abstract

Possible cardiac repair by adult stem cell transplantation is currently hampered by poor cell viability and delivery efficiency, uncertain differentiating fate in vivo, the needs of ex vivo cell expansion, and consequent delay in transplantation after the onset of heart attack. By the aid of magnetic resonance imaging, positron emission tomography, and immunohistochemistry, we show that injection of a hyaluronan mixed ester of butyric and retinoic acid (HBR) into infarcted rat hearts afforded substantial cardiovascular repair and recovery of myocardial performance. HBR restored cardiac [(18)F]fluorodeoxyglucose uptake and increased capillary density and led to the recruitment of endogenous Stro-1-positive stem cells. A terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling assay demonstrated that HBR-treated hearts exhibited a decrease in the number of apoptotic cardiomyocytes. In isolated rat cardiomyocytes and Stro-1 stem cells, HBR enhanced the transcription of vascular endothelial growth factor, hepatocyte growth factor, kdr, akt, and pim-1. HBR also increased the secretion of vascular endothelial growth factor and hepatocyte growth factor, suggesting that the mixed ester may have recruited both myocardial and...Continue Reading

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Citations

Jul 7, 2011·Stem Cell Reviews and Reports·Silvia AgostiniVincenzo Lionetti
Jul 31, 2013·Canadian Journal of Physiology and Pharmacology·Simone Lorenzo Romano, Vincenzo Lionetti
Jan 23, 2013·Vascular Pharmacology·Vincenzo Lionetti, Carlo Ventura
Jan 9, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Tommaso PrescimoneDaniela Giannessi
Aug 31, 2010·Translational Research : the Journal of Laboratory and Clinical Medicine·Vincenzo Lionetti, Fabio A Recchia
Nov 2, 2014·Journal of Biomedical Science·Francesca BonafèClaudio Muscari
Nov 22, 2011·Journal of Cellular and Molecular Medicine·Lucio Barile, Vincenzo Lionetti
May 23, 2012·Journal of Cellular and Molecular Medicine·Zhuzhi WenJingfeng Wang
May 29, 2014·Genesis : the Journal of Genetics and Development·Christopher ClowesKathryn E Hentges
Nov 13, 2014·Journal of Cellular and Molecular Medicine·Silvia AgostiniVincenzo Lionetti
Feb 5, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Yu-Wei ChiuYi-Lwun Ho
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Feb 22, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Manuela CampanFabio A Recchia
Oct 23, 2019·International Journal of Molecular Sciences·Silvia CanaiderCarlo Ventura
Dec 7, 2019·Minerva anestesiologica·Vincenzo Lionetti, Lucio Barile
Jul 12, 2019·World Journal of Stem Cells·Federica FacchinCarlo Ventura
Sep 17, 2019·World Journal of Stem Cells·Sara CrucianiMargherita Maioli
Dec 9, 2017·ACS Applied Materials & Interfaces·Shigang GuoXiaojun Peng

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Methods Mentioned

BETA
acylation
size exclusion chromatography
light microscopy
PCR
enzyme-linked immunosorbent assay
Histone Acetylation
acetylation
dissection

Software Mentioned

Image
MEDIS
NIS
Elements D Nikon
ECTB )
Mass®
Pro Plus®
GraphPad Prism

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