Hyaluronan molecular reflection by synovial lining is concentration dependent and reduced in dilute effusions in a rabbit model

Arthritis and Rheumatism
S SabaratnamJ R Levick

Abstract

Hyaluronan (HA) has a major role in regulating synovial fluid volume. This role depends on the synovium functioning as an ultrafilter that reflects HA during trans-synovial fluid drainage. Reflection boosts the HA concentration on the membrane surface, leading to osmotic retention of synovial fluid ("buffering"). In arthritic effusions, however, HA concentration and osmotic buffering are greatly reduced. We tested the hypothesis that reflection is reduced (escape increased) when the HA concentration falls below the molecular entanglement concentration (C*). HA at 0.2 mg/ml (<C*) or 1.5 mg/ml (>C*) was infused continuously into rabbit knee joints to set up a steady trans-synovial filtration. Joint-derived lymph was sampled over 3 hours, and subsynovial fluid was sampled at the end of the 3-hour period. HA was quantified by high-performance liquid chromatography to evaluate the reflected fraction. C* was determined by viscometry. Viscometry showed that 0.2 mg/ml HA was below C* and 1.5 mg/ml was above it. At 0.2 mg/ml, the mean +/- SEM HA reflected fraction was 0.66 +/- 0.04 (n = 7). At 1.5 mg/ml the reflection increased to 0.88 +/- 0.04 (n = 5) (P < 0.005). HA permeation increased almost 3-fold, from 12% to 34%, at the lower con...Continue Reading

References

Apr 1, 1989·Acta Orthopaedica Scandinavica·I ReimannE Svalastoga
Dec 1, 1987·Annals of the Rheumatic Diseases·D P Page-ThomasJ T Dingle
Jan 1, 1987·Arthritis and Rheumatism·W J WallisW B Nelp
Apr 8, 1998·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·T MurakamiY Nakanishi
Jul 15, 2000·The Journal of Physiology·P J ColemanJ R Levick
Mar 30, 2001·The Journal of Biological Chemistry·R PrevoD G Jackson
Jan 12, 2002·Current Opinion in Structural Biology·A J Day, J K Sheehan
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Shayn E Armstrong, Donald R Bell
May 27, 2003·The Journal of Physiology·C L AnggiansahJ R Levick
Oct 7, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Y LuW Wang
Oct 18, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·T S MombergerR M Mason

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