Hyaluronan promotes TRPV4-induced chondrogenesis in ATDC5 cells

PloS One
Yoshikazu OgawaToshihisa Kojima

Abstract

Hyaluronan (HA) is an extracellular matrix glycosaminoglycan essential for the homeostasis of cartilage-related tissues. Intracellular adhesion molecule-1 (ICAM-1) and CD44 have been identified as receptors for HA. Recently, transient receptor potential vanilloid 4 (TRPV4) has emerged as a potential research target in several areas of physiology. TRPV4 is a Ca2+-permeable, non-selective cation channel that appears to have mechanosensory or osmosensory roles in several musculoskeletal tissues. HA and TRPV4 play key roles in chondrogenesis; however, it has remained unclear whether they have interactive effects on chondrogenesis and, if so, how do they interact with each other? This study investigated the relationship between HA, its receptors ICAM-1 and CD44, and TRPV4 in the chondrogenic pathway using the ATDC5 cell line. It was found that the presence of HA is required for TRPV4-induced chondrogenesis. Loss of HA suppressed TRPV4-induced expression of the chondrogenic markers, SOX9 and Aggrecan. Moreover, HA affects TRPV4-induced chondrogenic development via each of ICAM-1 and CD44 partially. In conclusion, for the first time, the existence of an interaction between HA, its receptor ICAM-1 and CD44, and TRPV4-activity in chondr...Continue Reading

References

Feb 6, 2002·The Journal of Biological Chemistry·Hiroyuki WatanabeBernd Nilius
Apr 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R LEV, S S SPICER
Jul 2, 2004·Nature Reviews. Cancer·Bryan P Toole
Jul 4, 2007·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·T Yasuda
Sep 7, 2007·The Journal of Biological Chemistry·Shuji MuramatsuAkio Matsuda
Apr 16, 2010·Annals of the New York Academy of Sciences·Farshid GuilakWolfgang Liedtke
Dec 13, 2012·Biochemical and Biophysical Research Communications·Shinya HirabaraNaoki Ishiguro
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Christopher J O'ConorFarshid Guilak
Mar 4, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elizabeth C Lessey-MorillonKeith Burridge
May 27, 2014·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·John-Max MongkhonMohamed Benderdour
Sep 10, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Eiichi SatoHirotaka Haro
Apr 9, 2015·Quarterly Reviews of Biophysics·Harold A Scheraga

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