Hyaluronate accumulation and nerve-dependent growth during regeneration of larval Ambystoma limbs

Differentiation; Research in Biological Diversity
A L Mescher, C A Cox

Abstract

Hyaluronate-mediated expansion of the extracellular matrix has been suggested as an important element of growth and morphogenesis in several developing systems. In vitro, various growth factors have been shown to stimulate hyaluronate synthesis as well as cell proliferation. A similar link between proliferation and hyaluronate production during in vivo growth is difficult to demonstrate, because in most systems the source of growth-promoting factors is either not known or not amenable to experimental manipulation. During amphibian limb regeneration, cell proliferation depends upon paracrine release of factors from axons in the limb stump, and the nerve supply can be eliminated or augmented experimentally for study of growth in this system. Denervated and amputated limbs of larval salamanders do not begin to regenerate until distal areas of the limb stumps are reinnervated. We have used such limbs to examine the effect exerted by the reappearance of nerves on the amount of hyaluronate in the tissue undergoing the growth response. Hyaluronate was demonstrated by the metachromatic dye Ethyl Stains-all, which stains hyaluronate blue while sulfated glycosaminoglycans (GAGs) and proteins in the extracellular matrix stain various shad...Continue Reading

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Citations

Oct 12, 2001·Journal of Gastroenterology and Hepatology·J Y Fang, S D Xiao
May 16, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·A L MescherJ J Brokaw
Mar 15, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sarah Calve, Hans-Georg Simon
Feb 20, 2016·Developmental and Comparative Immunology·Anthony L MescherMichael W King
Jan 22, 2013·Proteomics·Catarina FrancoAna Varela Coelho
Feb 4, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·David L Stocum, Jo Ann Cameron
Mar 1, 1990·Differentiation; Research in Biological Diversity·S Filoni, L Paglialunga
Dec 15, 2010·Developmental Biology·Warnakulasuriya Akash FernandoKen Muneoka
Mar 20, 2013·Biomatter·Pouria Moshayedi, S Thomas Carmichael

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