Hyaluronate inhibits the interleukin-1beta-induced expression of matrix metalloproteinase (MMP)-1 and MMP-3 in human synovial cells

The Tohoku Journal of Experimental Medicine
Akiko SasakiMichiaki Takagi

Abstract

Intra-articular administration of hyaluronate (HA) is an effective treatment for arthritis. HA injections can decrease not only joint pain but also synovial effusion, although little is known concerning the mechanism of HA action. The aim of this study was to investigate the role of HA on the expression and production of matrix metalloproteinase (MMP) in synovial cells activated by interleukin (IL)-1beta in order to achieve a better understanding of exogenous HA function in the extracellular matrix degradation in arthritic joints. Human synovial cells were incubated with HA (0.1-1000 microg/ml) and/or IL-1beta (1 ng/ml). The expression of MMP-1 and MMP-3 mRNAs was analyzed by quantitative real-time polymerase chain reaction. The protein levels of MMP-1 and MMP-3 in cultured media were measured by immunoblotting. Expression of MMP-1 and MMP-3 mRNAs was induced by IL-1beta. The IL-1beta-mediated induction of MMP-1 mRNA expression was attenuated by 10 microg/ml HA (p=0.026) and that of MMP-3 mRNA was strongly down-regulated in the presence of 10 or 1000 microg/ml HA (p<0.001). The increased protein levels of MMP-1 and MMP-3 were also reduced by 1000 microg/ml HA. These data suggest that HA inhibits the expression and production of...Continue Reading

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Citations

Jan 16, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Hao PengBo Qiu
Jun 6, 2009·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Jian-lin ZhouHao Peng
Jan 12, 2007·Arthritis Research & Therapy·Kelly S SantangeloAlicia L Bertone
Oct 20, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Teng-Le HuangFeng-Huei Lin
Mar 20, 2018·Regenerative Medicine·Jehan J El-JawhariPeter V Giannoudis
Sep 22, 2017·Therapeutic Advances in Musculoskeletal Disease·Mohit BhandariJean-Pierre Pelletier
Aug 1, 2015·Revista Brasileira De Ortopedia·Alexandre Leme Godoy SantosBeat Hintermann
Jun 13, 2018·Revista Brasileira De Ortopedia·Marcello Zaia OliveiraLuiz Antonio Munhoz da Cunha
Mar 1, 2012·Revista Brasileira De Ortopedia·Márcia Uchôa de Rezende, Gustavo Constantino de Campos
Jul 12, 2019·Frontiers in Veterinary Science·Ramesh C GuptaAnita Sinha
Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Magdalena KusaczukMarzanna Cechowska-Pasko
Feb 27, 2021·Journal of Periodontal & Implant Science·Elena Sánchez-FernándezFrancisco Mesa

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