PMID: 9530997Apr 8, 1998Paper

Hyaluronectin is produced by oligodendrocytes and Schwann cells in vitro

Journal of Neurocytology
M N CourelB Delpech

Abstract

A hyaluronectin (HN)-like antigen was found in rat O-2A progenitors and oligodendrocytes, as well as in Schwann cells and in their culture medium. The HN-like antigen secreted in culture supernatants had a higher molecular mass than HN extracted from rat brain at acidic pH. In vitro the secreted HN-like antigen was spontaneously and slowly degraded into species whose Mr was close to that of HN found in acidic brain extract. In brain or nerve neutral pH extracts, both HN-like antigen and HN were present. The high Mr of the secreted antigen, the homology in amino acid sequences between HN and N-terminal domain of PG-M/versican, in addition to a positive hybridization between Schwann cell RNAs and a probe obtained with primers derived from HN sequences also found in versican suggested that HN is closely related to the large proteoglycan PG-M/versican. The presence in Schwann cell extract of a HN mRNA whose Mr was compatible with the size expected for HN showed that HN may be directly secreted by cells and not only the consequence of a proteolytic cleavage. The similarity of HN with PG-M (V3) suggested that HN found in vivo could be the result of an alternative splicing of a single gene. We conclude that HN as other members of the ...Continue Reading

Citations

Apr 10, 1999·The Journal of Biological Chemistry·R MiuraY Yamaguchi
Jul 22, 2005·Glia·Carmen Melendez-VasquezJames L Salzer
Apr 23, 2021·Developmental Biology·Zachary D RootDaniel M Medeiros
Oct 15, 2003·Neuron·James L Salzer
Jul 28, 2021·Proceedings of the National Academy of Sciences of the United States of America·Dhananjay YellajoshyulaWilliam T Dauer

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