Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation

Journal of Controlled Release : Official Journal of the Controlled Release Society
Tomoko ItoYoshiyuki Koyama

Abstract

Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasmid/PEI complexes in CHO cells.

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Citations

Mar 30, 2012·Biomacromolecules·Clark J NeedhamAntonios G Mikos
Nov 6, 2013·Biological & Pharmaceutical Bulletin·Tomoko Ito, Makoto Otsuka
Nov 26, 2008·Nanomedicine·Maria de la FuenteMaria Jose Alonso
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Jan 1, 2013·Colloids and Surfaces. B, Biointerfaces·Sonia Al-QadiCarmen Remunán-López
Oct 30, 2012·Colloids and Surfaces. B, Biointerfaces·Fatin Nawwab Al-DeenTim Williams
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Jul 16, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Keita UnMitsuru Hashida
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Feb 11, 2014·Carbohydrate Polymers·Thiruganesh RamasamyJong Oh Kim

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