Hyaluronic acid inhibits adhesion of hepatic stellate cells in spite of its stimulation of DNA synthesis

Tissue & Cell
Min Kyung ChoSang Geon Kim

Abstract

Unbalanced accumulation of fibers in extracellular matrix (ECM) results from attachment and activation of hepatic stellate cells (HSCs) during chronic liver diseases, in which the content of hyaluronic acid (HA), a glycosaminoglycan, in ECM changes. No information is available on the effect of HA on adhesion and activation of HSCs although that of collagen (Col) on HSCs was extensively studied. This study investigated the effects of HA with or without Col on adhesion of HSCs or the rate of DNA synthesis. Attachment of primary cultured HSCs was microscopically monitored in the plate simultaneously coated with HA or other ECM components. HA inhibited adhesion of quiescent HSCs at least up to 7 days after seeding, whereas HSCs were adherent to plastic or type I collagen (Col-I), type III collagen (Col-III), type IV collagen (Col-IV) or fibronectin. Both microscopy and alpha-smooth muscle actin immunocytochemistry revealed that the number of HSCs, which had been re-seeded after 15 days of culture, attached to HA-coated area was remarkably lower compared to that of HSCs on Col-I or plastic. Incorporation of HA into Col-I prevented adhesion of activated HSCs to matrix film. The number of HSCs adherent to HA at early times after seedi...Continue Reading

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Citations

Sep 3, 2014·European Journal of Pharmacology·Mona F MahmoudAmal Ibrahim
Nov 15, 2011·Toxicology and Applied Pharmacology·Eman M MantawyEbtehal El-Demerdash
Sep 29, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Raquel UrtasunNatalia Nieto
Oct 8, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ling ZhangXingdong Zhang
Mar 15, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Tannin J FujaIngo R Titze
Jun 2, 2016·Naunyn-Schmiedeberg's Archives of Pharmacology·Memy H Hassan, Mohamed M Ghobara
Mar 23, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi Jhen WuShyh Ming Kuo

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