Abstract
Unbalanced accumulation of fibers in extracellular matrix (ECM) results from attachment and activation of hepatic stellate cells (HSCs) during chronic liver diseases, in which the content of hyaluronic acid (HA), a glycosaminoglycan, in ECM changes. No information is available on the effect of HA on adhesion and activation of HSCs although that of collagen (Col) on HSCs was extensively studied. This study investigated the effects of HA with or without Col on adhesion of HSCs or the rate of DNA synthesis. Attachment of primary cultured HSCs was microscopically monitored in the plate simultaneously coated with HA or other ECM components. HA inhibited adhesion of quiescent HSCs at least up to 7 days after seeding, whereas HSCs were adherent to plastic or type I collagen (Col-I), type III collagen (Col-III), type IV collagen (Col-IV) or fibronectin. Both microscopy and alpha-smooth muscle actin immunocytochemistry revealed that the number of HSCs, which had been re-seeded after 15 days of culture, attached to HA-coated area was remarkably lower compared to that of HSCs on Col-I or plastic. Incorporation of HA into Col-I prevented adhesion of activated HSCs to matrix film. The number of HSCs adherent to HA at early times after seedi...Continue Reading
References
Feb 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S L FriedmanG Yamasaki
Jun 24, 1993·The New England Journal of Medicine·S L Friedman
Jun 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D VrochidesP Heldin
Jul 1, 1997·Journal of Internal Medicine·J R FraserU B Laurent
Aug 6, 1998·The Journal of Clinical Investigation·J P IredaleM J Arthur
Oct 23, 1998·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·H B RichterR Dargel
Mar 5, 1999·Breast Cancer Research and Treatment·M ToiT Tominaga
Sep 18, 2001·Gut·E J WilliamsJ P Iredale
Oct 5, 2001·Seminars in Liver Disease·D SchuppanE G Hahn
Oct 5, 2001·Seminars in Liver Disease·R C Benyon, M J Arthur
Oct 5, 2001·Seminars in Liver Disease·R Bataller, D A Brenner
Feb 8, 2002·Cell Structure and Function·K K TakaiS Irie
Feb 28, 2002·Journal of Hepatology·Marianna D A GaçaR Christopher Benyon
May 8, 2002·The Journal of Cell Biology·Boris HinzChristine Chaponnier
May 25, 2002·Liver·M T LevyM D Gorrell
Oct 23, 2002·Molecular Pharmacology·Keon Wook KangSang Geon Kim
Oct 25, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keon Wook KangSang Geon Kim
Jan 11, 2003·Cell Communication & Adhesion·Laura AlanizSilvia E Hajos
Feb 19, 2003·Tissue & Cell·Federica BoraldiIvonne Pasquali-Ronchetti
Apr 18, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Asifa S HaiderZbigniew Darzynkiewicz
Jun 17, 2003·Cell Structure and Function·Mitsuru SatoHaruki Senoo
Jun 19, 2003·British Journal of Pharmacology·Xuedong ChiChandrashekhar R Gandhi
Aug 12, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shih-Hsing LeirPeter M Lackie
Citations
Sep 3, 2014·European Journal of Pharmacology·Mona F MahmoudAmal Ibrahim
Nov 15, 2011·Toxicology and Applied Pharmacology·Eman M MantawyEbtehal El-Demerdash
Sep 29, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Raquel UrtasunNatalia Nieto
Oct 8, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ling ZhangXingdong Zhang
Mar 15, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Tannin J FujaIngo R Titze
Nov 7, 2013·Pharmaceutical Biology·Haiying DongYingcai Niu
Jun 2, 2016·Naunyn-Schmiedeberg's Archives of Pharmacology·Memy H Hassan, Mohamed M Ghobara
Mar 23, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi Jhen WuShyh Ming Kuo