Hyaluronic acid-modified bioadhesive liposomes as local drug depots: effects of cellular and fluid dynamics on liposome retention at target sites

Archives of Biochemistry and Biophysics
N Yerushalmi, Rimona Margalit

Abstract

Bioadhesive liposomes, in which hyaluronic acid is the surface-anchored bioadhesive ligand, are being tested in vitro in order to evaluate their bioadhesivity. The first part of the test, binding to monolayers of cells modeling the in vivo designated sites under static conditions, was reported in a previous communication. This communication reports the results of the second and third parts of the test, which consist of evaluating the retention of bound liposomes in the face of tissue-related events such as cell migration, proliferation, and death, and under fluid flow. The in vivo-designated binding sites for the bioadhesive liposomes were modeled, as before, by monolayers of the A431 cell line served. A setup for perfusing a culture flask containing a monolayer of cells was devised for measuring the retention under fluid flow. The major findings are: (1) Over a selected tested period of 28 h, the cell cultures mimicked the tissue-related events described above, whether they did or did not receive a dose of liposomes. Over the same period and throughout these events, the bioadhesive liposomes remained bound at equilibrium-like levels, in the range of 0.03 ng lipid/10(5) cells. (2) Fluid flowed over a cell monolayer dosed with b...Continue Reading

Citations

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