Hyaluronic Acid-Modified Porous Silicon Films for the Electrochemical Sensing of Bacterial Hyaluronidase.

Macromolecular Rapid Communications
Katrin-Stephanie TückingBeatriz Prieto-Simon

Abstract

The development of enzyme-responsive hyaluronic acid methacrylate (HYAMA)-coated porous silicon (pSi) films and their application in electrochemical diagnostic devices for the in situ detection of the enzyme hyaluronidase (hyal), which is secreted by Staphylococcus aureus (S. aureus) bacteria, are reported. The approach relies on a HYAMA-pSi electrode made of thermally hydrocarbonized pSi (pSi-THC) that is impregnated with crosslinked HYAMA/polyethylene glycol diacrylate (PEGDA) hydrogels. The enzymatic degradation of HYAMA by bacterial hyal is monitored by differential pulse voltammetry (DPV) utilizing pSi-THC as a working electrode and ferro/ferricyanide (FF) as external redox probe. The degradation of HYAMA results in reduced diffusion of the redox probe through the partially charged film, thereby enabling the detection of hyal by DPV. In addition to the determination of the concentration-dependent response in NaOAc buffer (pH 5.2), the detection of hyal as indicator for the presence of S. aureus bacteria above a threshold level in bacterial supernatants and artificial wound fluid is highlighted.

References

Mar 26, 2003·Biotechnology and Bioengineering·Jennie Baier LeachChristine E Schmidt
Jun 9, 2004·Microbiology·George MakrisKeith T Holland
Jan 1, 2005·Biosensors & Bioelectronics·Finny P Mathew, Evangelyn C Alocilja
Jan 11, 2005·Biomacromolecules·Jason A BurdickRobert Langer
Mar 9, 2006·Chemical Reviews·Robert Stern, Mark J Jedrzejas
May 25, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Roshan B VasaniNicolas H Voelcker
Jan 15, 2014·Biosensors & Bioelectronics·Jianlin Li, Michael J Sailor
May 6, 2015·Macromolecular Rapid Communications·Katrin-Stephanie TückingHolger Schönherr
Jun 13, 2015·Journal of the Royal Society, Interface·Alessandro CaliòLuca De Stefano

❮ Previous
Next ❯

Citations

Aug 17, 2019·Current Medicinal Chemistry·Javad TavakoliYouhong Tang
Nov 10, 2018·Analytical Chemistry·Sofia Arshavsky-GrahamSharon Weiss

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Hoppe-Seyler's Zeitschrift für physiologische Chemie
H GREILINGT EBERHARD
Duodecim; lääketieteellinen aikakauskirja
E KULONEN
Pharmaceutisch weekblad
P C HOEVENAARS
© 2022 Meta ULC. All rights reserved