Hyaluronidase enhancement of TNF-mediated cell death is reversed by TGF-beta 1

The American Journal of Physiology
N S Chang

Abstract

Both hyaluronidase and transforming growth factor (TGF)-beta 1 play a significant role in the development of prostate cancer. In this study, the regulation of tumor necrosis factor (TNF)-mediated cell death by hyaluronidase and TGF-beta 1 was investigated. Preexposure of L929 fibroblasts, prostate LNCaP cells, and epithelial Mv 1 Lu cells to hyaluronidase for a minimum of 12 h resulted in significant enhancement of cell death by TNF. Phosphorylation of p42 and p44 mitogen-activated-protein (MAP) kinases was found by stimulation of L929 cells with hyaluronidase for 30 min, indicating that the Raf/MAP kinase-extracellular signal-regulating protein kinase (MEK)/ MAP kinase pathway was activated. However, blocking the activation of upstream MAP kinase kinase (MEK 1 and 2 kinase) by PD-98059 failed to inhibit the hyaluronidase-enhanced TNF killing of cells, suggesting that hyaluronidase-mediated degradation of extracellular matrix and membrane components may elicit multiple signaling pathways. As a potent stimulator of extracellular matrix protein synthesis, TGF-beta 1 blocked the hyaluronidase-enhanced death of L929 and LNCaP cells mediated by TNF. TGF-beta 1 activated protein-tyrosine kinases in L929 cells, in which the tyrosine k...Continue Reading

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Citations

Jan 7, 2003·The Journal of Biological Chemistry·Nan-Shan ChangAmy Ensign
Jun 15, 2010·Experimental Biology and Medicine·Jean-Yun ChangNan-Shan Chang
Jul 25, 2009·Antioxidants & Redox Signaling·Yasuo IdoJoseph R Williamson
May 24, 2014·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·T Dylan OlverJ Kevin Shoemaker
Dec 22, 2016·Frontiers in Cell and Developmental Biology·Li-Jin HsuNan-Shan Chang
Jun 17, 2005·Journal of the Peripheral Nervous System : JPNS·James M Kennedy, Douglas W Zochodne
Dec 7, 2007·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·Effrossyni Georgakis, Roger Soames
Feb 13, 2001·Cancer Letters·G Lin, R Stern
May 29, 2007·European Journal of Pharmacology·Ljubica HarhajiVladimir Trajkovic

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