Hyaluronidase Enzyme-responsive Targeted Nanoparticles for Effective Delivery of 5-Fluorouracil in Colon Cancer

Pharmaceutical Research
Haiping JiangHaijun Lu

Abstract

In this study, we have successfully prepared the hyaluronic acid (HA)-conjugated mesoporous silica nanoparticles loaded with 5-fluorouracil (5-FU) to increase the anticancer efficacy in colon cancers. The particles were nanosized and perfectly spherical. In vitro release kinetics clearly showed the enzyme-sensitive release of 5-FU from HA-conjugated 5-FU loaded mesoporous silica nanoparticles (HA/FMSN). The presence of HA on the surface of nanoparticles targeted the CD44 receptors overexpressed in the colon cancer cells In vitro cell viability and apoptosis assay clearly showed the superior anticancer effect of HA/FMSN in HT29 colon cancer cells. HA/FMSN exhibited a remarkably higher 43% of cells in early apoptosis phase and 55% of cells in late apoptosis phase indicating the superior anticancer effect of HA/FMSN. HA/FMSN exhibited a significant reduction in the tumor burden compared to that of any group. HA/FMSN was 3-fold more effective than free drug and 2-fold more effective than -FU loaded mesoporous silica nanoparticles (FMSN). Overall, results suggest that the novel delivery strategy could hold enormous potential in colon cancer targeting.

References

May 2, 2003·Nature Reviews. Cancer·Daniel B LongleyPatrick G Johnston
Mar 9, 2006·Chemical Reviews·Robert Stern, Mark J Jedrzejas
Jan 11, 2011·Journal of the American Chemical Society·Chun-Ling ZhuXiao-Ru Wang
Apr 26, 2011·Nature Nanotechnology·Eun Chul ChoYounan Xia
Oct 5, 2011·ACS Nano·Ki Young ChoiJae Hyung Park
Mar 24, 2012·Chemical Society Reviews·Piaoping YangJun Lin
Jul 6, 2012·Critical Reviews in Oncology/hematology·Stephen J ClarkeJosep Tabernero
Aug 7, 2012·Current Gastroenterology Reports·Swati G Patel, Dennis J Ahnen
Oct 27, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Harmy Mohamed YusoffAmry Abdul Rahim
May 13, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·A AnithaR Jayakumar
Dec 3, 2014·Colloids and Surfaces. B, Biointerfaces·Xin PangGuangxi Zhai
Mar 6, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Cristina GiménezPedro Amorós
Aug 8, 2015·Chemistry : a European Journal·Simon GiretCarole Carcel
Nov 26, 2015·Advanced Drug Delivery Reviews·Franco DosioElias Fattal
Jan 27, 2016·World Journal of Gastroenterology : WJG·Arvind GulbakeSanjay K Jain
May 5, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Thiruganesh RamasamyJong Oh Kim

❮ Previous
Next ❯

Citations

Jul 26, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Firdos Alam KhanAbdelhamid Eliassari
Feb 28, 2019·Microscopy Research and Technique·Sultan AkhtarAbdullah Buhaimed
Apr 23, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Firdos Alam KhanAbdelhamid Elaissari
Sep 10, 2019·Therapeutic Delivery·Vahid Taghipour-SabzevarMehrdad Moosazadeh Moghaddam
Sep 14, 2018·International Journal of Biomaterials·Firdos Alam KhanSarah Ameen Almofty
Jul 2, 2019·Seminars in Cancer Biology·Eluri PavitraYun Suk Huh
Jan 28, 2021·Pharmaceutics·Eleen Dayana Mohamed IsaMartin R Gill
Jan 19, 2021·ACS Biomaterials Science & Engineering·Nan JiaMingxi Qiao

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
xenograft
dynamic light scattering
amidation

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Attention Disorders

Attention is involved in all cognitive activities, and attention disorders are reported in patients with various neurological diseases. Here are the latest discoveries pertaining to attention disorders.