Hyaluronidase-sensitive nanoparticle templates for triggered release of HIV/AIDS microbicide in vitro

The AAPS Journal
Vivek AgrahariBi-Botti C Youan

Abstract

This study was designed to test the hypothesis that a triggered release of a topical microbicide (tenofovir) from hyaluronic acid nanoparticles (HA-NPs) can be achieved under the influence of hyaluronidase (HAase) enzyme. A fractional factorial experimental design was used to examine the factors [molar concentrations of adipic acid dihydrazide (X1) and 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (X2), volume of acetone (X3) and reaction time (X4)] influencing the responses, Y1; particle mean diameter: PMD (nanometers: nm), Y2; polydispersity index: PDI and Y3; zeta (ζ) potential: (millivolts). The amide bond formation between HA and ADH after cross-linking was confirmed by FT-IR and (13)C-NMR analyses. These NPs were also characterized for cytotoxicity on a human vaginal epithelial cell line and L. crispatus. When formulated with factors X1; 2.49 mM, X2; 9.96 mM, X3; 60 mL, X4; 6 h, HA-NPs exhibited a spherical shape with PMD, PDI, ζ potential, encapsulation efficiency, and drug loading of 70.6 ± 4.1 nm, 0.07 ± 0.02, -38.2 ± 2.8 mV, 51.8 ± 2.4% w/w and 26.1 ± 1.2% w/w, respectively, (n = 3). Unlike for HA based gel, HAase significantly triggered the drug release and HA degradation from the NPs after 24 h (~90% ...Continue Reading

References

Apr 1, 1973·Journal of Reproduction and Fertility·C A FoxB W Watson
Sep 1, 1967·Archives of Biochemistry and Biophysics·M De SaleguiW Pigman
Jun 1, 1982·Annals of Internal Medicine·G Wagner, B Ottesen
Oct 1, 1994·Journal of Biomedical Materials Research·K M Lowry, E M Beavers
Apr 1, 1994·Biomaterials·S P ZhongD F Williams
May 1, 1997·Clinical and Diagnostic Laboratory Immunology·C Tevi-BénissanG Grésenguet
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·J E Scott, F Heatley
Jun 11, 1999·Contraception·D H Owen, D F Katz
Mar 27, 2001·Accounts of Chemical Research·G ZhangC Wu
Apr 12, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·P Costa, J M Sousa Lobo
Dec 4, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·A B CsokaR Stern
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR
Feb 3, 2004·Current Pharmaceutical Design·Osmond J D'Cruz, Fatih M Uckun
Jan 1, 1947·The Biochemical Journal·G I Swyer
Dec 13, 2006·Journal of Pharmaceutical Sciences·Kavita M GuptaPatrick F Kiser
Jun 5, 2007·Journal of Pharmaceutical Sciences·Sung-Up ChoiRodney J Y Ho
Aug 25, 2007·Biotechnology Advances·Robert SternLadislav Soltés
May 13, 2008·The Journal of Allergy and Clinical Immunology·Barton F Haynes, Robin J Shattock
Jan 8, 2009·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Katrin MoebusRoland Bodmeier
Feb 6, 2009·The AAPS Journal·Lisa Cencia Rohan, Alexandra B Sassi
Apr 22, 2009·AIDS·Brij B SaxenaSidney Lerner
Sep 18, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eun Ju OhSei Kwang Hahn
Oct 22, 2009·Journal of Nanobiotechnology·Raje Chouhan, Ak Bajpai
Nov 26, 2009·Human Reproduction Update·Trevor G CooperKirsten M Vogelsong
Mar 3, 2010·The AAPS Journal·Ahmed S ZidanMansoor A Khan
Apr 8, 2010·The AAPS Journal·Xiaowei MaXing-Jie Liang
Aug 6, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Yanzhuo ZhangSiling Wang
Aug 24, 2010·AAPS PharmSciTech·Pornsak SriamornsakSomkamol Manchun
Mar 26, 2011·Journal of Pharmaceutical Sciences·Dima AlukdaBi-Botti C Youan
Apr 26, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Glenn D Prestwich
Jun 28, 2011·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Jianing MengBi-Botti C Youan
Jun 28, 2011·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Rabeea F Omar, Michel G Bergeron
Jul 9, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Tao ZhangBi-Botti C Youan
Jul 13, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shoshy MizrahyDan Peer
Dec 20, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gaurav BajajYoon Yeo
Jan 5, 2012·AAPS PharmSciTech·Vivek Agrahari, Bi-Botti C Youan

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Citations

Feb 2, 2016·Advanced Drug Delivery Reviews·José das NevesBruno Sarmento
Oct 13, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ashim K MitraDhananjay Pal
Mar 25, 2016·Therapeutic Delivery·Vibhuti AgrahariAshim K Mitra
Apr 11, 2015·Advanced Drug Delivery Reviews·Sophia G Antimisiaris, Spyridon Mourtas
Jan 1, 2015·Advanced Drug Delivery Reviews·José das NevesBruno Sarmento
Nov 2, 2016·Molecular Pharmaceutics·Jianing MengBi-Botti C Youan
Dec 15, 2016·Expert Opinion on Drug Delivery·Vibhuti AgrahariAshim K Mitra
Jul 28, 2016·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jianing MengIbrahima Youm
Apr 16, 2015·Nanoscale·Rishi Rajat AdhikaryRinti Banerjee
Jun 28, 2018·AAPS PharmSciTech·Sonal Bhujbal, Alekha K Dash
Apr 13, 2017·Neural Regeneration Research·Vivek Agrahari
May 13, 2014·Drug Testing and Analysis·Vivek AgrahariBi-Botti C Youan
Aug 15, 2017·Biomaterials·Emily A KrogstadKim A Woodrow
Jul 3, 2021·Pharmaceutics·Tomasz OsmałekBozena Michniak-Kohn
Feb 2, 2016·Molecular Pharmaceutics·Vibhuti AgrahariAshim K Mitra

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