Hybrid Additive Microfabrication Scaffold Incorporated with Highly Aligned Nanofibers for Musculoskeletal Tissues

Tissue Engineering and Regenerative Medicine
Dilshan SooriyaarachchiGeorge Z Tan

Abstract

Latest tissue engineering strategies for musculoskeletal tissues regeneration focus on creating a biomimetic microenvironment closely resembling the natural topology of extracellular matrix. This paper presents a novel musculoskeletal tissue scaffold fabricated by hybrid additive manufacturing method. The skeleton of the scaffold was 3D printed by fused deposition modeling, and a layer of random or aligned polycaprolactone nanofibers were embedded between two frames. A parametric study was performed to investigate the effects of process parameters on nanofiber morphology. A compression test was performed to study the mechanical properties of the scaffold. Human fibroblast cells were cultured in the scaffold for 7 days to evaluate the effect of scaffold microstructure on cell growth. The tip-to-collector distance showed a positive correlation with the fiber alignment, and the electrospinning time showed a negative correlation with the fiber density. With reinforced nanofibers, the hybrid scaffold demonstrated superior compression strength compared to conventional 3D-printed scaffold. The hybrid scaffold with aligned nanofibers led to higher cell attachment and proliferation rates, and a directional cell organization. In addition...Continue Reading

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Citations

Jul 30, 2020·Tissue Engineering. Part B, Reviews·Anna AbbadessaMaría José Alonso
Sep 16, 2020·Materials·Cheol-Hwi Kim, Tae-Hyung Kim
Sep 26, 2020·Nanomedicine·Shawn P GroganDarryl D D'Lima
Dec 4, 2019·ACS Applied Materials & Interfaces·Mari Carmen EchaveManuela E Gomes

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