Hybrid Bioprinting of Chondrogenically Induced Human Mesenchymal Stem Cell Spheroids

Frontiers in Bioengineering and Biotechnology
Lise De MoorHeidi Declercq

Abstract

To date, the treatment of articular cartilage lesions remains challenging. A promising strategy for the development of new regenerative therapies is hybrid bioprinting, combining the principles of developmental biology, biomaterial science, and 3D bioprinting. In this approach, scaffold-free cartilage microtissues with small diameters are used as building blocks, combined with a photo-crosslinkable hydrogel and subsequently bioprinted. Spheroids of human bone marrow-derived mesenchymal stem cells (hBM-MSC) are created using a high-throughput microwell system and chondrogenic differentiation is induced during 42 days by applying chondrogenic culture medium and low oxygen tension (5%). Stable and homogeneous cartilage spheroids with a mean diameter of 116 ± 2.80 μm, which is compatible with bioprinting, were created after 14 days of culture and a glycosaminoglycans (GAG)- and collagen II-positive extracellular matrix (ECM) was observed. Spheroids were able to assemble at random into a macrotissue, driven by developmental biology tissue fusion processes, and after 72 h of culture, a compact macrotissue was formed. In a directed assembly approach, spheroids were assembled with high spatial control using the bio-ink based extrusion ...Continue Reading

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Citations

Dec 31, 2020·Cells·Océane MessaoudiAstrid Pinzano
Mar 2, 2021·Frontiers in Bioengineering and Biotechnology·Dimitrios Kouroupis, Diego Correa
Jan 30, 2021·Biofabrication·Marian FürsatzSylvia Nürnberger
May 20, 2021·Tissue Engineering. Part B, Reviews·Nicola C FosterAlicia J El Haj
Jun 26, 2021·Frontiers in Bioengineering and Biotechnology·Jessica NultyDaniel J Kelly
Aug 24, 2021·Frontiers in Bioengineering and Biotechnology·Esther Reina-RomoLiesbet Geris
Oct 8, 2021·Tissue Engineering and Regenerative Medicine·Guanhuier WangZhenmin Zhao
Oct 5, 2021·Tissue Engineering. Part B, Reviews·Sarah M Cooper, Roshni S Rainbow

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Methods Mentioned

BETA
bioprinting
chip
bioprinter
Light microscopy
Scanning Electron Microscopy

Software Mentioned

ImageJ
SPSS
Image J
BioCAD
Xcellence
Olympus

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