Hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
James A Reid, Anthony Callanan

Abstract

The aim when designing a scaffold is to provide a supportive microenvironment for the native cells, which is generally achieved by structurally and biochemically imitating the native tissue. Decellularized extracellular matrix (ECM) possesses the mechanical and biochemical cues designed to promote native cell survival. However, when decellularized and reprocessed, the ECM loses its cell supporting mechanical integrity and architecture. Herein, we propose dissolving the ECM into a polymer/solvent solution and electrospinning it into a fibrous sheet, thus harnessing the biochemical cues from the ECM and the mechanical integrity of the polymer. Bovine aorta and myocardium were selected as ECM sources. Decellularization was achieved using sodium dodecyl sulfate (SDS), and the ECM was combined with polycaprolactone and hexafluoro-2-propanol for electrospinning. The scaffolds were seeded with human umbilical vein endothelial cells (HUVECs). The study found that the inclusion of aorta ECM increased the scaffold's wettability and subsequently lead to increased HUVEC adherence and proliferation. Interestingly, the inclusion of myocardium ECM had no effect on wettability or cell viability. Furthermore, gene expression and mechanical chan...Continue Reading

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Citations

Jun 23, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·John A TerrellChengpeng Chen
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Fan ZhangMartin W King
Jun 30, 2021·Biofabrication·James Alexander ReidAnthony Callanan
Oct 10, 2021·Journal of Materials Science. Materials in Medicine·James Alexander ReidAnthony Callanan
Oct 30, 2021·Tissue Engineering and Regenerative Medicine·Büsra Baskapan, Anthony Callanan

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Methods Mentioned

BETA
infrared spectroscopy
electron scattering
fluorescence microscopy
PCR

Software Mentioned

LBADSA
[UNK] Spectra
ImageJ

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