Hybrid dynamic coating with n-dodecyl beta-D-maltoside and methyl cellulose for high-performance carbohydrate analysis on poly(methyl methacrylate) chips

Analytical Chemistry
Fuquan DangYoshinobu Baba

Abstract

Hybrid dynamic coating using n-dodecyl beta-d-maltoside (DDM) and methyl cellulose (MC) has been developed for suppression of analyte adsorption and electroosmotic flow (EOF) in a poly(methyl methacrylate) (PMMA) channel. The adsorption of APTS-labeled sugars in a PMMA channel was obviously suppressed with DDM dynamic coating; however, EOF was reduced only by a factor of approximately 25%, resulting in irreproducible separations. In contrast, both analyte adsorption and EOF in a PMMA channel were efficiently minimized with MC coating; however, concentrated MC above 0.3% was required to achieve high-performance separations, which greatly increased viscosity of the solution and caused difficulties during buffer loading and rinsing. In addition, n-dodecyltrimethylammonium chloride did not show observable effects on reducing analyte adsorption, although it has the same hydrophobic alkyl chain as DDM. These results strongly indicated that the polysaccharide moiety of surface modifiers has a specific affinity to surface charges and is crucial to achieving efficient and stable dynamic coating on the PMMA surface. Hybrid dynamic coating with 0.25% DDM and 0.03% MC was found to minimize both analyte adsorption and EOF in a PMMA channel ...Continue Reading

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Citations

Aug 19, 2011·Analytical and Bioanalytical Chemistry·M ViefhuesA Ros
Jun 9, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Nam Cao Hoai LeDavid E Williams
Jul 24, 2012·Electrophoresis·Glareh Azadi, Anubhav Tripathi
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May 23, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Fuquan DangYoshinobu Baba
Apr 9, 2013·Analytical Chemistry·Stefan MittermayrAndrás Guttman
Sep 16, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Mahmoud KhademiDominik P J Barz

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