Hybrid hydrogels self-assembled from HPMA copolymers containing peptide grafts

Macromolecular Bioscience
Jiyuan YangJ Kopecek

Abstract

Graft copolymers were designed that self-assemble into hydrogels mediated by the interaction of coiled-coil peptide domains. A linear hydrophilic polymer of HPMA was chosen as the backbone, and coiled-coil forming peptides, covalently attached to the backbone, formed the grafts. Microrheology was used to evaluate the self-assembly of graft copolymers into hydrogels. The results revealed that the length and the number of coiled-coil grafts per chain had a significant influence on the gelation process. At least 4 heptads were needed to achieve the association of graft copolymers into hydrogels. CD spectra of the copolymer containing 5 heptad grafts further suggested that coiled-coil formation may contribute to the self-assembly. Gelation of graft copolymers containing CC4 peptides indicated that a threshold amount of grafts per macromolecule is needed to form a three-dimensional structure. These studies demonstrated a potential of the graft copolymers to create self-assembling hydrogels with desirable and controllable structures.

References

Jun 24, 1991·Biochimica Et Biophysica Acta·M Jackson, H H Mantsch
Jan 1, 1995·Methods in Enzymology·R W Woody
Dec 1, 1996·Nature Structural Biology·L GonzalezT Alber
Feb 13, 1995·Physical Review Letters·T G Mason, D A Weitz
Jul 3, 1999·Nature·T MiyataT Uragami
Sep 6, 2000·Physical Review Letters·A J Levine, T C Lubensky
Sep 16, 2000·Physical Review Letters·J C CrockerD A Weitz
Oct 18, 2002·Advanced Drug Delivery Reviews·Y Bruce Yu
Sep 19, 2003·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Jindrich Kopecek
Mar 15, 2005·Nature Materials·Jason D EhrickSylvia Daunert

❮ Previous
Next ❯

Citations

Jun 7, 2008·Chemical Communications : Chem Comm·Marc A Gauthier, Harm-Anton Klok
Apr 28, 2010·Chemical Society Reviews·Congqi Yan, Darrin J Pochan
Feb 14, 2013·Applied Physics Letters·Christopher J Rowlands, Peter T C So
Oct 28, 2008·Acta Biomaterialia·Jindrich Kopecek, Jiyuan Yang
Sep 24, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Te-Wei ChuJindřich Kopeček
Aug 19, 2007·Biomaterials·Jindrich Kopecek
Aug 2, 2008·Progress in Polymer Science·Somali ChaterjiKinam Park
Jun 27, 2015·Biomacromolecules·Ulla I M Gerling-DriessenL Hartmann
Jan 24, 2012·Biotechnology Advances·Michal Pechar, Robert Pola
Aug 14, 2018·Macromolecular Bioscience·Elham Radvar, Helena S Azevedo
Oct 16, 2008·Macromolecular Bioscience·Larisa Cristina RaduJindrich Kopecek
Jan 22, 2010·Biopolymers·Ohm D Krishna, Kristi L Kiick
Feb 11, 2010·Macromolecular Bioscience·Jiyuan YangJindrich Kopecek
May 18, 2010·Angewandte Chemie·Hana Robson Marsden, Alexander Kros
Jun 27, 2019·Organic & Biomolecular Chemistry·Ronja Otter, Pol Besenius
Jul 19, 2012·Angewandte Chemie·Jindřich Kopeček, Jiyuan Yang
Aug 15, 2012·Chemical Society Reviews·Eric A AppelOren A Scherman
May 24, 2008·Biomacromolecules·Ying ZhaoTianwei Tan
Jan 23, 2008·Biomacromolecules·Jiyuan YangJindrich Kopecek
Sep 12, 2009·Biomacromolecules·Erinc Sahin, Kristi L Kiick
Nov 5, 2008·Journal of the American Chemical Society·Weiwei YuanJindrich Kopecek
Oct 22, 2008·Biomacromolecules·Bojana Apostolovic, Harm-Anton Klok
Mar 24, 2018·Advanced Drug Delivery Reviews·Robert DimatteoTatiana Segura
Feb 5, 2009·Bioconjugate Chemistry·Albert J de GraafEnrico Mastrobattista
May 25, 2012·Journal of the American Chemical Society·Zhen ChenZachary T Ball
Apr 25, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Laurent J GoujonHarald D H Stöver
Jan 12, 2011·Macromolecules·Bradley D OlsenDavid A Tirrell
Jul 30, 2019·Biomacromolecules·Lindsay K HillJin Kim Montclare

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.