Hybrid Model Membrane Combining Micropatterned Lipid Bilayer and Hydrophilic Polymer Brush

Langmuir : the ACS Journal of Surfaces and Colloids
Toshiki NishimuraKenichi Morigaki

Abstract

Substrate-supported planar lipid bilayers (SPBs) are being utilized as a versatile model system of the biological membrane. However, the proximity between the solid support and membrane limits utility of SPBs for the functional analyses of membrane proteins. Here, we present a model membrane that can enlarge the distance between the substrate surface and the membrane by combining a stable scaffold of polymerized lipid bilayer with a hydrophilic polymer brush. A micropatterned SPB was generated by the lithographic polymerization of diacetylene lipids and subsequent incorporation of natural (fluid) lipid bilayers. Hydrophilic polymer brush of poly-2-methacryloyloxyethyl phosphorylcholine (poly(MPC)) was formed on the surface of polymeric bilayer by the in situ atom transfer radical polymerization (ATRP) in aqueous solution, in the presence of embedded fluid lipid bilayers. A model membrane protein (Haloquadratum walsbyi bacteriorhodopsin: HwBR) could be reconstituted into the polymer brush-supported bilayers with significantly reduced immobile molecules. Furthermore, the polymer brush terminals could be functionalized by successively polymerizing MPC and 2-aminoethyl methacrylate (AMA). The reactive amine moiety of poly(AMA) enab...Continue Reading

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Citations

Jun 6, 2018·Chemical Society Reviews·Xiangcheng PanKrzysztof Matyjaszewski
Jan 19, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kenichi Morigaki

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