Hybrid phase ligation for efficient synthesis of histone proteins

Organic & Biomolecular Chemistry
Ruixuan R YuJennifer J Ottesen

Abstract

We introduce a hybrid solid-solution phase ligation approach that combines the efficiency of solid phase ligation with solution phase ligation in the total synthesis of modified histone proteins. A two linker strategy allows analysis throughout work on the solid phase and maximizes yields through cleavage at an external Rink, while an internal HMBA linker allows the native carboxyl terminus for any protein sequence. We demonstrate this approach for two histone proteins: triple-acetylated H4-K5ac, K12ac, K91ac and CENP-A-K124ac.

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Citations

Apr 20, 2016·The Journal of Organic Chemistry·Ignacio ColomerAlma Viso
Apr 12, 2017·Epigenetics & Chromatin·Minh BuiYamini Dalal
Jan 31, 2017·Chemical Communications : Chem Comm·Yuya AsahinaHironobu Hojo
Sep 2, 2017·Chemical Communications : Chem Comm·Nora GuidottiBeat Fierz
Aug 3, 2018·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Takuma SueokaAkimitsu Okamoto
Feb 24, 2018·Frontiers in Chemistry·Yun-Kun QiBaihui Yan
Nov 6, 2020·Organic & Biomolecular Chemistry·Tianjiong YaoScott K Silverman
Nov 3, 2020·Journal of the American Chemical Society·Ganga B VamisettiAshraf Brik
Apr 4, 2020·Current Opinion in Chemical Biology·Vangelis AgouridasOleg Melnyk
Jun 8, 2021·Angewandte Chemie·Skander A AbboudVincent Aucagne
Nov 9, 2019·Bioconjugate Chemistry·Jennifer BouchennaOleg Melnyk
Aug 17, 2017·Biochemistry·Takuma SueokaAkimitsu Okamoto

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