Hybrid-primed lymphocytes and hybrid vaccination prevent tumor growth of lewis lung carcinoma in mice

Journal of Immunotherapy
Rajkumar SavaiGamal-Andre Banat

Abstract

Dendritic cell (DC)-tumor cell hybrids are currently being evaluated as a novel antitumor vaccination strategy. We have explored in an animal model whether administration of DCs fused with poorly immunogenic carcinoma cells could elicit an antitumor response. Fusion of C57/BL6 mice bone marrow-derived DCs with Lewis lung carcinoma (LLC1) cells resulted in approximately 50% fusion efficiency. Hybrid cells (HCs) were used to explore 3 potential tumor therapy strategies: protective immunization, vaccination, and adoptive cellular therapy. Immunization with HCs induced activation of proliferating cytotoxic T cells, upregulation of distinct cytokines genes, and a significant retardation of tumor growth. Similar results were observed by vaccination with HCs in the tumor-bearing host. Finally, when T cells from HC-vaccinated mice were transferred into naive tumor-bearing mice, tumor growth was strongly retarded and an efficient proliferative and cytotoxic T-cell response was observed. Tumor growth was reduced by more than 50%, and tumor development was significantly delayed. Taken together, we demonstrate that HCs offer effective immunotherapy of poorly immunogenic carcinomas. This is independent of whether the HCs are taken for adopt...Continue Reading

References

Jan 1, 1991·Annual Review of Immunology·R M Steinman
Jan 17, 1996·Journal of the National Cancer Institute·N P RestifoS A Rosenberg
Aug 1, 1996·The Journal of Experimental Medicine·D BoczkowskiE Gilboa
Feb 1, 1997·Current Opinion in Immunology·M CellaA Lanzavecchia
Mar 17, 1997·International Journal of Cancer. Journal International Du Cancer·S K NairE Gilboa
Jun 1, 1997·Immunology Today·T BoonB Van den Eynde
Feb 12, 1998·The Journal of Experimental Medicine·G Schuler, R M Steinman
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Apr 29, 1998·Cancer Immunology, Immunotherapy : CII·E GilboaH K Lyerly
Mar 12, 1999·Annual Review of Medicine·J M Timmerman, R Levy
Jul 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J GongD Kufe
Feb 28, 2001·Cancer Immunology, Immunotherapy : CII·G A BanatM Zöller
Jan 31, 2002·World Journal of Gastroenterology : WJG·J ZhangH B Chen
Jul 26, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Takashi HayashiSuyu Shu
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Petra Kleindienst, Thomas Brocker
Apr 25, 2003·International Journal of Cancer. Journal International Du Cancer·Masaya KawadaTakashi Yoshiki
Apr 30, 2003·Cellular Immunology·Hiroshi TanakaSuyu Shu
Nov 25, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Leon T van den BroekeJay A Berzofsky
Dec 31, 2003·Cellular Immunology·Jorgen KjaergaardSuyu Shu

❮ Previous
Next ❯

Citations

Aug 10, 2007·Journal of Translational Medicine·Jin YuSebastiano Gattoni-Celli
May 5, 2011·Journal of Biomedicine & Biotechnology·Shigeo KoidoHisao Tajiri
Feb 9, 2011·Cytotherapy·Dominique CathelinBernard Bonnotte
Apr 10, 2010·Journal of Biomedicine & Biotechnology·Shigeo KoidoHisao Tajiri
Nov 5, 2010·Clinical & Developmental Immunology·Shigeo KoidoHisao Tajiri
Mar 12, 2013·Human Vaccines & Immunotherapeutics·Michael J Browning
Feb 26, 2010·Clinical & Developmental Immunology·Shigeo KoidoHisao Tajiri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.