Hybrid transcription system for controlled plastid transgene expression

The Plant Journal : for Cell and Molecular Biology
Laurence BuhotSilva Lerbs-Mache

Abstract

Plastid transformation technologies have developed rapidly over the last few years, reflecting their value in the study of the principal mechanisms of plastid gene expression and commercial interest in using plastids as bioreactors. Application of this technology is still limited by the difficulty of obtaining regulated, selective expression of plastid transgenes. The plastid genome is transcribed by two different types of RNA polymerase. One of them is of the eubacterial type of polymerase, and its subunits are encoded in the plastid genome [plastid-encoded RNA polymerase (PEP)]. The other one is of the phage type and nucleus-encoded [nucleus-encoded RNA polymerase (NEP)]. To obtain selective transgene expression, we have made use of the similarities and differences between the eubacterial and the plastid eubacterial type transcription systems. We created a hybrid transcription system in which the transgene is placed under the control of a eubacterial promoter which does not exist in the plastid genome and which is not recognized by the plastid endogenous transcriptional machinery. Selective transcription of the transgene is achieved by the supply of a chimeric transcription factor that interacts with PEP and directs it specif...Continue Reading

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Citations

Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andreas VerhounigRalph Bock
Nov 20, 2012·Journal of Experimental Botany·Maureen R HansonBeth A Ahner
Oct 25, 2012·Journal of Biomedicine & Biotechnology·Meili GaoJiangang Long
Aug 17, 2011·Biotechnology Advances·Nunzia ScottiTeodoro Cardi
Dec 5, 2015·Plant & Cell Physiology·Christian R BoehmJim Haseloff
Feb 24, 2007·Current Opinion in Biotechnology·Stephen P MayfieldJulia Marin-Navarro
Jul 25, 2007·Plant Biotechnology Journal·Denis J Murphy
Mar 31, 2011·Plant Biotechnology Journal·Andreas G Lössl, Mohammad T Waheed
Sep 26, 2006·Trends in Biotechnology·Muhammad Sarwar Khan
Oct 9, 2013·Biotechnology Letters·N Scotti, T Cardi
Nov 5, 2016·Journal of Experimental Botany·Niaz AhmadPeter J Nixon
Aug 3, 2010·Expert Review of Vaccines·Teodoro CardiPal Maliga

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