Hybridization of genes involved in aflatoxin biosynthesis to DNA of aflatoxigenic and non-aflatoxigenic aspergilli

Applied Microbiology and Biotechnology
M A KlichThomas E Cleveland

Abstract

Southern blots of DNA from a number of aspergilli belonging to Aspergillus section Flavi, including aflatoxin-producing and non-aflatoxigenic isolates of A. flavus and A. parasiticus, were probed with the aflatoxin pathway genes aflR and omt-1. DNA of all A. flavus, A. parasiticus and A. sojae isolates examined hybridized with both genes. None of the A. oryzae isolates examined hybridized to the aflR probe and one of the three did not hybridize to the omt-1 probe. None of the A. tamarii isolates examined hybridized to either gene. Our results suggest that some isolates in this section do not produce aflatoxin because they lack at least one of the genes necessary for biosynthesis, and that non-producing A. flavus, A. parasiticus and A. sojae strains either lack a gene we did not examine or have genes that are not being expressed.

Citations

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Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.