PMID: 8604306Feb 15, 1996Paper

Hybridization properties of oligodeoxynucleotide pairs bridged by polyarginine peptides

Nucleic Acids Research
Z WeiS Stein

Abstract

The hybridization properties of a series of probes, based on two 9mer oligodeoxynucleotides (designated as I and II) having an appended oligoarginine chain (Rn) to produce peptide-oligonucleotide conjugates or peptide-bridged oligonucleotide pairs (e.g. Rn-I or II-Rn-I), were investigated. For the double-linked probes, we found that the peptide bridge induces the two 9mers to bind complementary single-stranded DNA or RNA targets with substantially enhanced thermal stability. The resulting hybrid with complementary DNA was found to assume a 1:1 complex in the B conformation as judged by UV mixing curves and CD spectroscopy. Complexes of single or double-linked probes with complementary RNA exhibited sensitivity to RNase H digestion. The influence of the identity and chirality of the repeating unit in the bridge, the length of the bridge, the gap size and the salt concentration on the hybridization properties of this new class of oligonucleotide probes was also studied. Our data reveal that these compounds exhibit properties that should prove useful in the development of antisense strategies.

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C H TungS Stein
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Tao, A D Frankel
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G E PlumK J Breslauer
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Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K J BreslauerL A Marky
Mar 14, 1967·Journal of Molecular Biology·D E OlinsP H Von Hippel
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Jan 1, 1993·Antisense Research and Development·T ZhuS Stein
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Citations

Jan 5, 1999·Bioorganic & Medicinal Chemistry Letters·D A SarracinoC Richert
May 5, 2011·Journal of the American Chemical Society·Keith T GagnonDavid R Corey
Jun 5, 2007·Nature Protocols·Emilie VoirinMitsuharu Kotera
Jul 29, 2006·Chembiochem : a European Journal of Chemical Biology·Bénédicte PonsJean-Paul Behr
Nov 27, 2010·Chembiochem : a European Journal of Chemical Biology·Merita MurtolaRoger Strömberg
Feb 22, 2005·Advanced Drug Delivery Reviews·Hilary BrooksEric Vivès
Jan 3, 2019·Drug Development and Industrial Pharmacy·Arehalli S Manjappa, Rayasa S Ramachandra Murthy
Jan 24, 2003·The Journal of Biological Chemistry·Alam Nur-E-KamalLeroy F Liu
May 6, 2010·Drug Metabolism Reviews·Federica VacondioBernard Testa
Sep 20, 2000·Bioconjugate Chemistry·C H Tung, S Stein

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