Hybridoma growth in a new generation hollow fibre bioreactor: antibody productivity and consistency.

Cytotechnology
N KesslerM Aymard

Abstract

This paper analyses the performance of MAbMax(TM)/Tricentric(TM), a new generation hollow fibre bioreactor, for hybridoma growth and antibody productivity, the down stream processing of monoclonal antibody harvests throughout the run and the further control of antibody quality consistency. Handling and process parameters were optimised using a mouse hybridoma, IgG1(K) secretor, and then confirmed with several other hybridomas. Cells were kept at optimal viability during an unusually long period of time and a continuously high production of antibodies was detected over several months. Foetal bovine serum concentration was reduced to 1\% and the effects of weaning of cells from serum were monitored in terms of cell metabolism and antibody productivity. Antibody harvests collected at regular intervals throughout the run (2 to 12 weeks) were purified using affinity chromatography on a recombinant protein A/G matrix and then analysed in terms of antigen binding properties, isoelectric forms and oligosaccharide structures, in order 1) to control antibody quality consistency as a function of time and serum concentration and 2) to compare antibody characteristics as a function of culture conditions, in vitro bioreactor cultivation vers...Continue Reading

References

Nov 5, 1992·Journal of Immunological Methods·J M Coco-MartinE C Beuvery
Dec 15, 1992·Biochemical and Biophysical Research Communications·H TachibanaH Murakami
Mar 1, 1993·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·N KesslerM Aymard
Mar 1, 1986·Biotechnology and Bioengineering·J P Tharakan, P C Chau

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Citations

Mar 18, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·O P DancetteP Blond
Jul 23, 2009·Biotechnology and Bioengineering·Rachel LegmannSeth T Rodgers
Oct 9, 2007·Biotechnology Advances·Era Jain, Ashok Kumar
Oct 3, 2021·Cold Spring Harbor Protocols·Edward A Greenfield

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