Hybridomas: a new dimension in biological analyses

In Vitro
R H Kennett

Abstract

Since the first report of hybridomas producing monoclonal antibodies by Kohler and Milstein in 1975, this technique has spread to nearly all areas of biological, biochemical, and biomedical research. Watching the use of these methods spread from immunologists to cell biologists, developmental biologists, biochemists and to other biological disciplines and observing the nearly logarithmic increase in publications using these reagents has been in itself fascinating and informative. An overview of the development of this technology and its applications is presented including the use of monoclonal antibodies to study cell surface molecules, differentiation antigens, receptors, and histocompatibility antigens. The use of these antibodies to analyze microorganisms and parasitic antigens as well as their use in the genetic analysis of human cell surface antigens and the detection of polymorphic variation in enzymes and other proteins is discussed. Examples of the application of monoclonal reagents to the study of tumor cell biology including the labeling of metastatic tumor cells and the detection of cell surface molecules implicated in the regulation of growth control and cell division are provided.

References

Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·N R KlinmanP J Gearhart
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H KoprowskiM Herlyn
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M PierresR N Germain
Jan 1, 1979·Immunological Reviews·F M BrodskyW F Bodmer
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W C RaschkeM E Koshland
Jul 14, 1977·Nature·S Gillis, K A Smith
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K B BechtolR H Kennett
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P N GoodfellowH O McDevitt
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M HerlynH Koprowski
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M Y YehK E Hellström
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T F LinsenmayerC D Little
Aug 3, 1978·Nature·S KontiainenM Feldmann
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E L ReinherzS F Schlossman
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D L GasserR H Kennett
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R LevyR Warnke
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A C CuelloC Milstein
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G S EisenbarthM Nirenberg
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D J Charron, H O McDevitt
Jan 1, 1979·Immunological Reviews·J A Ledbetter, L A Herzenberg
Jan 1, 1979·Immunological Reviews·T J McKearnF P Stuart
May 28, 1979·Biochemical and Biophysical Research Communications·C M GomezF W Fitch
Jun 1, 1979·The Australian Journal of Experimental Biology and Medical Science·G F MitchellM C Howard
Jan 1, 1979·Journal of Supramolecular Structure·J J StarlingK D Noonan
Jul 1, 1976·European Journal of Immunology·G Köhler, C Milstein

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Citations

Feb 1, 1985·European Journal of Clinical Microbiology·H Krakauer
Feb 18, 2010·Applied Biochemistry and Biotechnology·Sina SarikhaniTooran Mirshahi
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·N I Goldstein, R H Cagan
Jan 26, 2006·BMC Bioinformatics·Hao-Teng ChangMargaret Dah-Tsyr Chang
Jul 12, 2012·Oncology Letters·Botao NingHongqiang Shen

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