Hydration analysis of Pseudomonas aeruginosa cytochrome c551 upon acid unfolding by dielectric relaxation spectroscopy

Biophysical Chemistry
Tetsuichi WazawaMakoto Suzuki

Abstract

Dielectric relaxation (DR) study was performed to reveal the hydration change of Pseudomonas aeruginosa ferric cytochrome c551 (PA c551) in dilute aqueous solutions upon the acid unfolding which undergoes a two-state transition. The DR spectrum of a small spherical region containing a PA c551 molecule and its surrounding water shell was derived from the solution and solvent spectra by dielectric mixture theories. The derived spectrum was well-fitted with a sum of a Debye relaxation component (C1) with a DR frequency around 4.7GHz and the bulk solvent component (CB). Upon acid unfolding, the DR amplitude of CB decreased with decreasing pH in an inverse manner to that of C1, while the total DR amplitude was almost constant. It indicates that C1 is due to the hydration water of PA c551. Little change in the DR frequency of C1 and a 1.7-fold increase in hydration number were observed.

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Citations

Dec 20, 2012·The Journal of Chemical Physics·Yoji KubotaRyo Akiyama
Jun 23, 2016·The Journal of Physical Chemistry. B·Makoto SuzukiTetsuichi Wazawa
Sep 21, 2016·Bioscience, Biotechnology, and Biochemistry·Misa MasanariYoshihiro Sambongi
Mar 21, 2017·Bioscience, Biotechnology, and Biochemistry·Shinya KobayashiYoshihiro Sambongi
Nov 1, 2017·Cytoskeleton·Makoto SuzukiNobuyuki Matubayasi

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