Hydration of rainbow trout oocyte during meiotic maturation and in vitro regulation by 17,20{beta}-dihydroxy-4-pregnen-3-one and cortisol

The Journal of Experimental Biology
Sylvain MillaJulien Bobe

Abstract

Although oocytes of many teleost fish, especially marine species, are subjected to a hydration process during meiotic maturation, which leads to an important volume increase, no noticeable hydration of the preovulatory oocyte has ever been reported in rainbow trout (Oncorhynchus mykiss). In the present study, oocyte water content and dry mass were monitored using consecutive samples taken in vivo from the same female rainbow trout, from 4-5 days prior to ovulation to up to 7 days post-ovulation. In addition, yolk protein electrophoretic patterns were compared between oocytes sampled prior to germinal vesicle breakdown (GVBD) and unfertilized eggs. Furthermore, the effect of the maturation-inducing steroid (17,20beta-dihydroxy-4-pregnen-3-one, 17,20beta-P), cortisol and 11-deoxycorticosterone (DOC) on oocyte dry and wet masses, as well as GVBD occurrence was assessed in vitro. Finally, mRNA expression profiles of glucocorticoid and mineralocorticoid receptors as well as 11beta-hydroxysteroid dehydrogenase (11beta-HSD) were monitored in the periovulatory ovary by real-time PCR. Both in vivo and in vitro data showed, for the first time in rainbow trout, that a significant oocyte hydration occurs during oocyte maturation. In additi...Continue Reading

References

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Citations

Jun 5, 2010·Archives of Environmental Contamination and Toxicology·S NadzialekPatrick Kestemont
Sep 13, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Rachel J WebbAnthony E Michael
Oct 20, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Mao LiJohn F Leatherland
Feb 27, 2010·Ontogenez·M N Skoblina
Aug 27, 2010·Journal of Fish Biology·J F LeatherlandS Barkataki
Mar 21, 2006·General and Comparative Endocrinology·P PrunetS Milla
Nov 26, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Julien BobeAlexis Fostier
Mar 21, 2021·General and Comparative Endocrinology·Andre BaranyStephen D McCormick
Oct 13, 2015·Theriogenology·Nathalia Nogueira da CostaOtávio Mitio Ohashi
Sep 5, 2021·Fish Physiology and Biochemistry·Jin Ah SongCheol Young Choi
Mar 26, 2009·General and Comparative Endocrinology·Constantinos C MylonasSilvia Zanuy
Jun 10, 2009·General and Comparative Endocrinology·Esther LubzensJoan Cerdà

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